Rabbit anti-Human DKK3 Polyclonal Antibody | anti-DKK3 antibody

DKK3, NT (Dickkopf-related Protein 3, Dickkopf-3, Dkk-3, REIC, NQ258/PRO295)

Cat. Num. AAA626275

• Gene Names: DKK3; RIG; REIC
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: DKK3; Polyclonal Antibody; NT (Dickkopf-related Protein 3; Dickkopf-3; Dkk-3; REIC; NQ258/PRO295); Anti -DKK3; anti-DKK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DKK3.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-DKK3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human DKK3 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with DKK3 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with DKK3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(DKK3 Antibody (A30) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the DKK3 antibody detected the DKK3 protein (arrow).)

Related Product Information for anti-DKK3 antibody

DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.

Product Categories/Family for anti-DKK3 antibody

Antibodies; Abs to Dickkopf Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 66346689
• NCBI GeneID: 27122
• NCBI Accession #: NP_001018067.1
• NCBI GenBank Nucleotide #: NM_001018057.1
• UniProt Accession #: Q6PQ81; Q9UBP4
• Molecular Weight: 38,340 Da
• NCBI Official Full Name: dickkopf-related protein 3
• NCBI Official Synonym Full Names: dickkopf homolog 3 (Xenopus laevis)
• NCBI Official Symbol: DKK3
• NCBI Official Synonym Symbols: RIG; REIC
• NCBI Protein Information: dickkopf-related protein 3; dkk-3; hDkk-3; dickkopf 3; dickkopf-3; RIG-like 5-6; RIG-like 7-1; OTTHUMP00000230783; OTTHUMP00000230784; OTTHUMP00000230785; OTTHUMP00000230786; regulated in glioma
• UniProt Protein Name: Putative tumor suppressor Dkk-3/REIC
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK3
• UniProt Entry Name: Q6PQ81_HUMAN

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Uniprot Description

DKK3: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular space

Biological Process: anatomical structure morphogenesis; Wnt receptor signaling pathway; adrenal gland development; negative regulation of aldosterone biosynthetic process; negative regulation of transcription, DNA-dependent