Rabbit anti-Human Dkk-2 CT Polyclonal Antibody | anti-DKK2 antibody

Dkk-2 CT (Dickkopf Related Protein-2, Dickkopf-2, DKK-2, UNQ682/PRO1316)

Cat. Num. AAA631051

• Gene Names: DKK2; DKK-2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Dkk-2 CT; Polyclonal Antibody; Dkk-2 CT (Dickkopf Related Protein-2; Dickkopf-2; DKK-2; UNQ682/PRO1316); Anti -Dkk-2 CT (Dickkopf Related Protein-2; anti-DKK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DKK2.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-DKK2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Peptide corresponding to a sequence selected for the C-terminal region of human DKK2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DKK2 Antibody (C239) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the DKK2 antibody detected the DKK2 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-DKK2 antibody

The 259aa DKK2 protein, like DKK1, DKK3, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cys residues each. The second cys region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.

Product Categories/Family for anti-DKK2 antibody

Antibodies; Abs to Dickkopf Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 7657023
• NCBI GeneID: 27123
• NCBI Accession #: NP_055236.1
• NCBI GenBank Nucleotide #: NM_014421.2
• UniProt Accession #: Q9UBU2; Q9UIU3; A0AVE9; B2R6S7; Q9H3R7
• Molecular Weight: 28,447 Da
• NCBI Official Full Name: dickkopf-related protein 2
• NCBI Official Synonym Full Names: dickkopf homolog 2 (Xenopus laevis)
• NCBI Official Symbol: DKK2
• NCBI Official Synonym Symbols: DKK-2
• NCBI Protein Information: dickkopf-related protein 2; hDkk-2; dickkopf 2; dickkopf-2; OTTHUMP00000161876; dickkopf related protein-2
• UniProt Protein Name: Dickkopf-related protein 2
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK2
• UniProt Entry Name: DKK2_HUMAN

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). [provided by RefSeq]

Uniprot Description

DKK2: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP5 and LRP6. Expressed in heart, brain, skeletal muscle and lung. Belongs to the dickkopf family.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space

Biological Process: Wnt receptor signaling pathway; multicellular organismal development