Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Brain Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Brain Tissue.)
Rabbit anti-Rat Dihydrolipoyl Dehydrogenase Polyclonal Antibody | anti-DLD antibody
Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Brain Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Brain Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant DLD, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant DLD, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Adrenal Gland Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Adrenal Gland Tissue)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. A similar protein in human, mouse, and pig has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, the protein can function as a protease. [provided by RefSeq, Jan 2014]
Uniprot Description
DLD: a multi-functional mitochondrial enzyme. An enzymatic component of the mitochondrial glycine cleavage system, the pyruvate dehydrogenase complex (PDHC), the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Is the E3 component of the PDHC that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. The E3 component has dihydrolipoamide dehydrogenase activity. The PDHC contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. Defects in DLD are a cause of maple syrup urine disease (MSUD), characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation. Differentially expressed in the Wernicke's Area from patients with schizophrenia. Inhibited by 5-methoxyindole-2-carboxylic acid (MICA).
Protein type: Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; EC 1.8.1.4; Mitochondrial; Oxidoreductase
Chromosomal Location of Human Ortholog: 6q16
Cellular Component: acrosomal matrix; cilium; mitochondrial matrix; mitochondrion; myelin sheath; nucleoplasm; oxoglutarate dehydrogenase complex; pyruvate dehydrogenase complex
Molecular Function: dihydrolipoyl dehydrogenase activity; electron transfer activity; FAD binding; lipoamide binding; NAD binding
Biological Process: 2-oxoglutarate metabolic process; acetyl-CoA biosynthetic process from pyruvate; aging; cell redox homeostasis; dihydrolipoamide metabolic process; gastrulation; lipoate metabolic process; mitochondrial electron transport, NADH to ubiquinone; proteolysis; regulation of membrane potential; sperm capacitation
Research Articles on DLD
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Product Notes
The DLD dld (Catalog #AAA2027119) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Dihydrolipoyl Dehydrogenase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-5ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the DLD dld for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dihydrolipoyl Dehydrogenase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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