Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Serum; Lane2: Rat Pancreas Tissue; Lane3: Rat Brain Tissue; Lane4: Rat Adrenal Gland Tissue.)
Rabbit anti-Rat Branched Chain Alpha-Ketoacid Dehydrogenase Kinase Polyclonal Antibody | anti-BCKDK antibody
Polyclonal Antibody to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Serum; Lane2: Rat Pancreas Tissue; Lane3: Rat Brain Tissue; Lane4: Rat Adrenal Gland Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Serum; Lane2: Rat Pancreas Tissue; Lane3: Rat Brain Tissue; Lane4: Rat Adrenal Gland Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant BCKDK, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant BCKDK, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Heart Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Heart Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue)
NCBI and Uniprot Product Information
NCBI Description
mitochondrial kinase; involved in oxidative degradation of branched-chain amino acids [RGD, Feb 2006]
Uniprot Description
BCKDK: an atypical protein kinase associated with the mitochondrial matrix. Contains a HATPase_c domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. Unlike PHKs, BCK dimerizes through direct interaction of two opposing nucleotide-binding domains. Phosphorylates and inactivates the E1-alpha subunit of the branched-chain ketoacid dehydrogenase (BCKDK), the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Inactivating mutations have been discovered in BCKDK in families with autism, epilepsy, and intellectual disability, a syndrome that may respond to dietary supplementation.
Protein type: ATYPICAL group; EC 2.7.11.4; Kinase, protein; Mitochondrial; PDHK family; Protein kinase, atypical
Chromosomal Location of Human Ortholog: 1q37
Cellular Component: mitochondrial alpha-ketoglutarate dehydrogenase complex; mitochondrion
Molecular Function: [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase activity; ATP binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity, transferring phosphorus-containing groups
Biological Process: branched-chain amino acid catabolic process; phosphorylation; protein amino acid phosphorylation
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Product Notes
The BCKDK bckdk (Catalog #AAA2028641) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Branched Chain Alpha-Ketoacid Dehydrogenase Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the BCKDK bckdk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Branched Chain Alpha-Ketoacid Dehydrogenase Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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