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Western Blot (WB) (Western Blot: Sample: Mouse Placenta lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse DAO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Mouse Diamine Oxidase Polyclonal Antibody | anti-DAO antibody

Polyclonal Antibody to Diamine Oxidase (DAO)

Gene Names
Aoc1; DAO; Abp1; 1600012D06Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Diamine Oxidase; Polyclonal Antibody; Polyclonal Antibody to Diamine Oxidase (DAO); anti-DAO antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-DAO antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-3ug/mL
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Mouse
Immunogen
Recombinant ABP1 (Arg197~Trp322 (Accession # Q8JZQ5) ) expressed in E.coli (MBS2011702)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Mouse Placenta lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse DAO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Placenta lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse DAO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant DAO, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant DAO, Mouse.)

Western Blot (WB)

(Western BlotSample: Mouse Small intestine lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse DAO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Western Blot (WB) (Western BlotSample: Mouse Small intestine lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse DAO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Mouse Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P. Samples: Mouse Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Amiloride-sensitive amine oxidase
NCBI Official Synonym Full Names
amine oxidase, copper-containing 1
NCBI Official Symbol
Aoc1
NCBI Official Synonym Symbols
DAO; Abp1; 1600012D06Rik
NCBI Protein Information
amiloride-sensitive amine oxidase [copper-containing]
UniProt Protein Name
Amiloride-sensitive amine oxidase [copper-containing]
Protein Family
UniProt Gene Name
Aoc1
UniProt Synonym Gene Names
Abp1; DAO; Diamine oxidase

Uniprot Description

ABP1: Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function. Belongs to the copper/topaquinone oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - histidine; Amino Acid Metabolism - tryptophan; EC 1.4.3.22; Oxidoreductase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6 B2.3|6 23.78 cM

Cellular Component: bicellular tight junction; extracellular region; extracellular space; plasma membrane

Molecular Function: amine oxidase activity; calcium ion binding; copper ion binding; drug binding; heparin binding; metal ion binding; oxidoreductase activity; protein complex binding; protein homodimerization activity; quinone binding; receptor activity; zinc ion binding

Biological Process: amiloride transport; amine metabolic process; response to antibiotic; response to drug

Research Articles on DAO

Similar Products

Product Notes

The DAO aoc1 (Catalog #AAA2026318) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Diamine Oxidase (DAO) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Diamine Oxidase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). Western blotting: 0.5-3ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the DAO aoc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Diamine Oxidase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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