Rabbit anti-Human DIABLO Polyclonal Antibody | anti-DIABLO antibody

DIABLO Antibody

Cat. Num. AAA9404381

• Gene Names: DIABLO; SMAC; DFNA64
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: DIABLO; Polyclonal Antibody; DIABLO Antibody; Diablo; IAP-binding mitochondrial protein; SMAC; SMAC3; DFNA64; DIABLO-S; anti-DIABLO antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total DIABLO protein.
• Form/Format: Supplied at 2.2mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 1362

• Applicable Applications for anti-DIABLO antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:2000-1:10000; Western blotting: 1:1000-1:5000; Immunohistochemistry: 1:100-1:300
• Immunogen Type: Recombinant protein
• Immunogen Description: Fusion protein corresponding to a region derived from 56-239 amino acids of human diablo, IAP-binding mitochondrial protein
• Target Name: DIABLO
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 12%SDS-PAGELane1: Lncap cell lysateLane2: SKOV3 cell lysateLane3: MCF7 cell lysateLane4: 293T cell lysateLysates: 40 ug per lanePrimary antibody: 1/1000 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 1 second)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using 31125 (DIABLO Antibody) at dilution 1/70, on the right is treated with the fusion protein.)

Related Product Information for anti-DIABLO antibody

This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.

Product Categories/Family for anti-DIABLO antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 40225836
• NCBI GeneID: 56616
• Molecular Weight: 22,284 Da
• NCBI Official Full Name: Homo sapiens diablo homolog (Drosophila), mRNA
• NCBI Official Synonym Full Names: diablo, IAP-binding mitochondrial protein
• NCBI Official Symbol: DIABLO
• NCBI Official Synonym Symbols: SMAC; DFNA64
• NCBI Protein Information: diablo homolog, mitochondrial
• UniProt Protein Name: Diablo homolog, mitochondrial
• Protein Family: Diablo
• UniProt Gene Name: DIABLO
• UniProt Synonym Gene Names: SMAC; Smac
• UniProt Entry Name: DBLOH_HUMAN

NCBI Description

This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]

Uniprot Description

DIABLO: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. Homodimer. Interacts with NGFRAP1/BEX3. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin. Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: internal side of plasma membrane; mitochondrion; mitochondrial intermembrane space; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; neuron apoptosis; induction of apoptosis via death domain receptors; positive regulation of apoptosis; apoptosis; caspase activation via cytochrome c; induction of apoptosis by oxidative stress

Disease: Deafness, Autosomal Dominant 64