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Western Blot (WB) (Western blotAll lanes: DHX35 antibody at 12 ug/mlLane 1: Mouse kidney tissueLane 2: Mouse liver tissueLane 3: K562 whole cell lysateLane 4: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 79,76 kDaObserved band size: 79 kDa)

Rabbit anti-Human, Mouse DHX35 Polyclonal Antibody | anti-DHX35 antibody

DHX35 Antibody

Gene Names
DHX35; DDX35; C20orf15; KAIA0875
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%,Protein G purified
Synonyms
DHX35; Polyclonal Antibody; DHX35 Antibody; Probable ATP-dependent RNA helicase DHX35; DEAH box protein 35; C20orf15; DDX35; anti-DHX35 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%,Protein G purified
Form/Format
Liquid
Applicable Applications for anti-DHX35 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB:1:1000-1:5000
IHC:1:20-1:200
Immunogen
Recombinant Human Probable ATP-dependent RNA helicase DHX35 protein (1-300AA)
Conjugate
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Species
Homo sapiens (Human)
Research Area
Epigenetics and Nuclear Signaling
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-85524
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: DHX35 antibody at 12 ug/mlLane 1: Mouse kidney tissueLane 2: Mouse liver tissueLane 3: K562 whole cell lysateLane 4: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 79,76 kDaObserved band size: 79 kDa)

Western Blot (WB) (Western blotAll lanes: DHX35 antibody at 12 ug/mlLane 1: Mouse kidney tissueLane 2: Mouse liver tissueLane 3: K562 whole cell lysateLane 4: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 79,76 kDaObserved band size: 79 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human brain tissue using MBS7000394 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissue using MBS7000394 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7000394 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS7000394 at dilution of 1:100)
Related Product Information for anti-DHX35 antibody
May be involved in pre-mRNA splicing.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
probable ATP-dependent RNA helicase DHX35 isoform 2
NCBI Official Synonym Full Names
DEAH-box helicase 35
NCBI Official Symbol
DHX35
NCBI Official Synonym Symbols
DDX35; C20orf15; KAIA0875
NCBI Protein Information
probable ATP-dependent RNA helicase DHX35
UniProt Protein Name
Probable ATP-dependent RNA helicase DHX35
UniProt Gene Name
DHX35
UniProt Synonym Gene Names
C20orf15; DDX35
UniProt Entry Name
DHX35_HUMAN

NCBI Description

DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of this gene product which is a member of this family, has not been determined. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

DHX35: May be involved in pre-mRNA splicing. Belongs to the DEAD box helicase family. DEAH subfamily.

Protein type: RNA splicing; Spliceosome; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 20q11.22-q12

Molecular Function: ATP binding; ATP-dependent RNA helicase activity

Biological Process: nuclear mRNA splicing, via spliceosome; RNA processing

Research Articles on DHX35

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Product Notes

The DHX35 dhx35 (Catalog #AAA7000394) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHX35 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DHX35 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB:1:1000-1:5000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the DHX35 dhx35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHX35, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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