Rabbit anti-Human DHX30 Polyclonal Antibody | anti-DHX30 antibody

DHX30, NT (DHX30, DDX30, KIAA0890, Putative ATP-dependent RNA helicase DHX30, DEAH box protein 30) (Azide free) (HRP)

Cat. Num. AAA6292095

• Gene Names: DHX30; DDX30; RETCOR
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DHX30; Polyclonal Antibody; NT (DHX30; DDX30; KIAA0890; Putative ATP-dependent RNA helicase DHX30; DEAH box protein 30) (Azide free) (HRP); DEAH box protein 30; anti-DHX30 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DHX30 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DHX30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human DHX30.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DHX30 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the DHX30 antibody detected the DHX30 protein (arrow).)

Related Product Information for anti-DHX30 antibody

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein has 97% sequence identity with the mouse HELG protein. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Product Categories/Family for anti-DHX30 antibody

Antibodies; DEAH Box Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Zhou, Y., et al. Virology 372(1):97-106(2008). Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008). Takezawa, S., et al. EMBO J. 26(3):764-774(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 20336290
• NCBI GeneID: 22907
• NCBI Accession #: NP_055781.2; NP_619520.1
• NCBI GenBank Nucleotide #: NM_014966.3
• UniProt Accession #: Q7L2E3; O94965; Q7Z753; Q96CH4; Q9NUQ0; A8K5F1
• Molecular Weight: 129,438 Da
• NCBI Official Full Name: putative ATP-dependent RNA helicase DHX30 isoform 2
• NCBI Official Synonym Full Names: DEAH (Asp-Glu-Ala-His) box helicase 30
• NCBI Official Symbol: DHX30
• NCBI Official Synonym Symbols: DDX30; RETCOR
• NCBI Protein Information: putative ATP-dependent RNA helicase DHX30
• UniProt Protein Name: Putative ATP-dependent RNA helicase DHX30
• Protein Family: Putative ATP-dependent RNA helicase
• UniProt Gene Name: DHX30
• UniProt Synonym Gene Names: DDX30; KIAA0890
• UniProt Entry Name: DHX30_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]

Uniprot Description

DDX30: Required for optimal function of the zinc-finger antiviral protein ZC3HAV1. Associates with mitochondrial DNA. Belongs to the DEAD box helicase family. DEAH subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; EC 3.6.4.13; RNA-binding; Helicase

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; double-stranded RNA binding; chromatin binding; ATP binding; ATP-dependent RNA helicase activity

Biological Process: RNA processing