Rabbit DHX30 Polyclonal Antibody | anti-DHX30 antibody

DHX30 Antibody

Cat. Num. AAA9630056

• Gene Names: DHX30; DDX30; RETCOR
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DHX30; Polyclonal Antibody; DHX30 Antibody; anti-DHX30 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: DHX30 Antibody detects endogenous levels of DHX30.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-DHX30 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human DHX30.
• Conjugation: Unconjugated
• Similarity: Belongs to the DEAD box helicase family. DEAH subfamily.
• Post Translational Modifications: Phosphorylated on Ser-15.
• Subunit Structure: Identified in a complex with TFAM and SSBP1. Interacts with AGO1 and AGO2. Interacts (via N-terminus) with ZC3HAV1 (via N-terminal domain) in an RNA-independent manner (By similarity). Found in a complex with GRSF1, DDX28, FASTKD2 and FASTKD5.
• Function: RNA-dependent helicase. Plays an important role in the assembly of the mitochondrial large ribosomal subunit. Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (By similarity). Associates with mitochondrial DNA. Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD (By similarity).
• Note: Localizes to mitochondrial RNA granules found in close proximity to the mitochondrial nucleoids (PubMed:16825194, PubMed:25683715). Relocalizes to stress granules upon heat stress (PubMed:29100085).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-DHX30 antibody

Cytoplasm; Mitochondrion; Mitochondrion matrix; Mitochondrion nucleoid; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 20336290
• NCBI GeneID: 22907
• NCBI Accession #: NP_055781.2
• NCBI GenBank Nucleotide #: NM_014966.3
• UniProt Accession #: Q7L2E3; O94965; Q7Z753; Q96CH4; Q9NUQ0; A8K5F1
• Molecular Weight: 129,438 Da
• NCBI Official Full Name: putative ATP-dependent RNA helicase DHX30 isoform 2
• NCBI Official Synonym Full Names: DEAH (Asp-Glu-Ala-His) box helicase 30
• NCBI Official Symbol: DHX30
• NCBI Official Synonym Symbols: DDX30; RETCOR
• NCBI Protein Information: putative ATP-dependent RNA helicase DHX30
• UniProt Protein Name: Putative ATP-dependent RNA helicase DHX30
• Protein Family: Putative ATP-dependent RNA helicase
• UniProt Gene Name: DHX30
• UniProt Synonym Gene Names: DDX30; KIAA0890
• UniProt Entry Name: DHX30_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]

Uniprot Description

DDX30: Required for optimal function of the zinc-finger antiviral protein ZC3HAV1. Associates with mitochondrial DNA. Belongs to the DEAD box helicase family. DEAH subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; EC 3.6.4.13; RNA-binding; Helicase

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; double-stranded RNA binding; chromatin binding; ATP binding; ATP-dependent RNA helicase activity

Biological Process: RNA processing