Western Blot (WB)
(Western blot analysis of SPRYD2 in mouse heart tissue lysate with SPRYD2 antibody at 1 μg/mL.)
Rabbit SPRYD2 Polyclonal Antibody | anti-CMYA5 antibody
SPRYD2 Antibody
Gene Names
CMYA5; SPRYD2; TRIM76; C5orf10
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
SPRYD2 Antibody is affinity chromatography purified via peptide column.
Synonyms
SPRYD2; Polyclonal Antibody; SPRYD2 Antibody; TRIM76; C5orf10; DTNBP2; Cardiomyopathy-associated protein 5; Dystrobrevin-binding protein 2; cardiomyopathy associated 5; anti-CMYA5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SPRYD2 antibody is predicted to not cross-react with other SPRYD protein family members. At least four isoforms of SPRYD2 are known to exist.
Purity/Purification
SPRYD2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
4069
Applicable Applications for anti-CMYA5 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
SPRYD2 antibody can be used for detection of SPRYD2 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
SPRYD2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD2.
Buffer
SPRYD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SPRYD2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of SPRYD2 in mouse heart tissue lysate with SPRYD2 antibody at 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of SPRYD2 in mouse heart tissue lysate with SPRYD2 antibody at 1 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 20 μg/mL.)
Related Product Information for anti-CMYA5 antibody
SPRYD2 Antibody: SPRYD2, also known as Myospryn, was originally identified as the muscle-specific partner of dysbindin and as a Mef-2 target gene. It is a large scaffolding protein localized to the Z-disc/costamere region of striated muscle. SPRYD2 includes a noncanonical tripartite motif (TRIM-like) that lacks the RING domain but consists of a B-box coiled coil (BBC), fibronectin 3 (FN3) repeats, and SPRY domains. SPRYD2 interacts with desmin and calcineurin, and it has been suggested to play a role in the biogenesis of lysosome and negatively regulates slow-fiber-type transformation and skeletal muscle regeneration. SPRYD2 is dysregulated in Duchenne muscular dystrophy.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
449,211 Da
NCBI Official Full Name
cardiomyopathy-associated protein 5
NCBI Official Synonym Full Names
cardiomyopathy associated 5
NCBI Official Symbol
CMYA5
NCBI Official Synonym Symbols
SPRYD2; TRIM76; C5orf10
NCBI Protein Information
cardiomyopathy-associated protein 5; myospryn; genethonin-3; 2310076E16Rik; dystrobrevin-binding protein 2; tripartite motif-containing 76; SPRY domain-containing protein 2; tripartite motif-containing protein 76
UniProt Protein Name
Cardiomyopathy-associated protein 5
Protein Family
UniProt Gene Name
CMYA5
UniProt Synonym Gene Names
C5orf10; DTNBP2; SPRYD2; TRIM76
UniProt Entry Name
CMYA5_HUMAN
Uniprot Description
CMYA5: May serve as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) via binding to PRKAR2A. May function as a repressor of calcineurin-mediated transcriptional activity. May attenuate calcineurin ability to induce slow-fiber gene program in muscle and may negatively modulate skeletal muscle regeneration.
Chromosomal Location of Human Ortholog: 5q14.1
Cellular Component: costamere; perinuclear region of cytoplasm; M band
Molecular Function: identical protein binding; protein binding
Biological Process: negative regulation of protein phosphatase type 2B activity; regulation of skeletal muscle adaptation
Research Articles on CMYA5
Similar Products
Product Notes
The CMYA5 cmya5 (Catalog #AAA150809) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPRYD2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SPRYD2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). SPRYD2 antibody can be used for detection of SPRYD2 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the CMYA5 cmya5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPRYD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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