Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using DHRS3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Rabbit anti-Mouse, Rat DHRS3 Polyclonal Antibody | anti-DHRS3 antibody
DHRS3 Polyclonal Antibody
Gene Names
DHRS3; SDR1; RDH17; Rsdr1; DD83.1; SDR16C1; retSDR1
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Synonyms
DHRS3; Polyclonal Antibody; DHRS3 Polyclonal Antibody; DD83.1; RDH17; retSDR1; Rsdr1; SDR1; SDR16C1; anti-DHRS3 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
PAKLRDLSRENVLITGGGRGIGRQLAREFAERGARKIVLWGRTEKCLKETTEEIRQMGTECHYFICDVGNREEVYQTAKAVREKVGDITILVNNAAVVHGKSLMDSDDDALLKSQHINTLG
Sequence Length
302
Applicable Applications for anti-DHRS3 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human DHRS3
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Membrane, Multi-pass membrane protein
Positive Samples
Mouse kidney, Rat kidney
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using DHRS3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using DHRS3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Product Categories/Family for anti-DHRS3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 18kDa; 33kDa
Observed: 33kDa
Observed: 33kDa
NCBI Official Full Name
short-chain dehydrogenase/reductase 3 isoform 1
NCBI Official Synonym Full Names
dehydrogenase/reductase 3
NCBI Official Symbol
DHRS3
NCBI Official Synonym Symbols
SDR1; RDH17; Rsdr1; DD83.1; SDR16C1; retSDR1
NCBI Protein Information
short-chain dehydrogenase/reductase 3
UniProt Protein Name
Short-chain dehydrogenase/reductase 3
Protein Family
UniProt Gene Name
DHRS3
UniProt Synonym Gene Names
RDH17; SDR16C1; retSDR1
NCBI Description
Short-chain dehydrogenases/reductases (SDRs), such as DHRS3, catalyze the oxidation/reduction of a wide range of substrates, including retinoids and steroids (Haeseleer and Palczewski, 2000 [PubMed 10800688]).[supplied by OMIM, Jun 2009]
Uniprot Description
Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.
Research Articles on DHRS3
Similar Products
Product Notes
The DHRS3 dhrs3 (Catalog #AAA9134182) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHRS3 Polyclonal Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DHRS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the DHRS3 dhrs3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PAKLRDLSRE NVLITGGGRG IGRQLAREFA ERGARKIVLW GRTEKCLKET TEEIRQMGTE CHYFICDVGN REEVYQTAKA VREKVGDITI LVNNAAVVHG KSLMDSDDDA LLKSQHINTL G. It is sometimes possible for the material contained within the vial of "DHRS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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