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Western Blot (WB) (DHRS3 Antibody (Center) western blot analysis in HepG2 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the DHRS3 antibody detected the DHRS3 protein (arrow).)

Rabbit DHRS3 Polyclonal Antibody | anti-DHRS3 antibody

DHRS3 Antibody (Center)

Gene Names
DHRS3; SDR1; RDH17; Rsdr1; DD83.1; SDR16C1; retSDR1
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DHRS3; Polyclonal Antibody; DHRS3 Antibody (Center); Short-chain dehydrogenase/reductase 3; DD831; Retinal short-chain dehydrogenase/reductase 1; retSDR1; anti-DHRS3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DHRS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-113 amino acids from the Central region of human DHRS3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
85-113
Sequence Length
167
Applicable Applications for anti-DHRS3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(DHRS3 Antibody (Center) western blot analysis in HepG2 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the DHRS3 antibody detected the DHRS3 protein (arrow).)

Western Blot (WB) (DHRS3 Antibody (Center) western blot analysis in HepG2 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the DHRS3 antibody detected the DHRS3 protein (arrow).)

Western Blot (WB)

(Western blot analysis of DHRS3 (arrow) using rabbit polyclonal DHRS3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHRS3 gene.)

Western Blot (WB) (Western blot analysis of DHRS3 (arrow) using rabbit polyclonal DHRS3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHRS3 gene.)
Related Product Information for anti-DHRS3 antibody
DHRS3 catalyze the oxidation/reduction of a wide range of substrates, including retinoids and steroids.
Product Categories/Family for anti-DHRS3 antibody
References
Persson,B., et.al., Chem. Biol. Interact. 178 (1-3), 94-98 (2009)
Roni,V., et.al., BMC Genomics 8, 42 (2007)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33548
NCBI Official Full Name
short-chain dehydrogenase/reductase 3
NCBI Official Synonym Full Names
dehydrogenase/reductase (SDR family) member 3
NCBI Official Symbol
DHRS3
NCBI Official Synonym Symbols
SDR1; RDH17; Rsdr1; DD83.1; SDR16C1; retSDR1
NCBI Protein Information
short-chain dehydrogenase/reductase 3
UniProt Protein Name
Short-chain dehydrogenase/reductase 3
UniProt Gene Name
DHRS3
UniProt Synonym Gene Names
RDH17; SDR16C1; retSDR1
UniProt Entry Name
DHRS3_HUMAN

NCBI Description

Short-chain dehydrogenases/reductases (SDRs), such as DHRS3, catalyze the oxidation/reduction of a wide range of substrates, including retinoids and steroids (Haeseleer and Palczewski, 2000 [PubMed 10800688]).[supplied by OMIM, Jun 2009]

Uniprot Description

DHRS3: Catalyzes the reduction of all-trans-retinal to all- trans-retinol in the presence of NADPH. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 1.1.1.300; Membrane protein, multi-pass; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p36.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: electron carrier activity; nucleotide binding; retinol dehydrogenase activity

Biological Process: negative regulation of retinoic acid receptor signaling pathway; phototransduction, visible light; visual perception; retinol metabolic process; palate development; retinoid metabolic process; regulation of ossification

Research Articles on DHRS3

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Product Notes

The DHRS3 dhrs3 (Catalog #AAA9203269) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 85-113. The DHRS3 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's DHRS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the DHRS3 dhrs3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHRS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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