Immunohistochemistry (IHC)
(MBS9602255 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human TDE1 Polyclonal Antibody | anti-TDE1 antibody
TDE1 Antibody
Gene Names
SERINC3; TDE; TDE1; AIGP1; TMS-1; DIFF33; SBBI99
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TDE1; Polyclonal Antibody; TDE1 Antibody; AIGP1; DIFF33; placental transmembrane protein (mouse testicular differentially expressed); SBBI99; serine incorporator 3; TDE; TMS 1; Transmembrane protein SBBI99; Tumor differentially expressed 1; Tumor differentially expressed protein 1; Tumour differentially expressed 1; anti-TDE1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
TDE1 antibody detects endogenous levels of total TDE1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
473
Applicable Applications for anti-TDE1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IHC: 1:200
IF/ICC: 1:100-1:500
IHC: 1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human TDE1
Subcellular Location
Cell Membrane; Multipass Membrane Protein
Tissue Specificity
Ubiquitous. Expression levels were increased fourfold to tenfold in lung tumor tissues compared with normal pulmonary tissues.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (89%), Bovine (89%), Horse (100%), Sheep (89%), Rabbit (89%), Dog (100%), Xenopus (88%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9602255 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9602255 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of TDE1 using 293 whole cell lysates)
Western Blot (WB)
(Western blot analysis of TDE1 using 293 whole cell lysates)
Immunofluorescence (IF)
(MBS9602255 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9602255 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-TDE1 antibody
Function: Restriction factor required to restrict infectivity of lentiviruses, such as HIV-1: acts by inhibiting an early step of viral infection. Impairs the penetration of the viral particle into the cytoplasm (PubMed:26416733, PubMed:26416734).
Post-translational Modifications: N-glycosylated.
Similarity: Belongs to the TDE1 family.
Post-translational Modifications: N-glycosylated.
Similarity: Belongs to the TDE1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 52 kDa
Predicted: 53 kDa
Predicted: 53 kDa
NCBI Official Full Name
serine incorporator 3
NCBI Official Synonym Full Names
serine incorporator 3
NCBI Official Symbol
SERINC3
NCBI Official Synonym Symbols
TDE; TDE1; AIGP1; TMS-1; DIFF33; SBBI99
NCBI Protein Information
serine incorporator 3
UniProt Protein Name
Serine incorporator 3
UniProt Gene Name
SERINC3
UniProt Synonym Gene Names
DIFF33; TDE11 PublicationManual assertion based on opinion iniRef.1
Uniprot Description
Restriction factor required to restrict infectivity of lentiviruses, such as HIV-1: acts by inhibiting an early step of viral infection. Impairs the penetration of the viral particle into the cytoplasm (PubMed:26416733, PubMed:26416734).
Research Articles on TDE1
1. SERINC3 and SERINC5 together restricted HIV-1 replication, and this restriction was evaded by Nef
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Product Notes
The TDE1 serinc3 (Catalog #AAA9602255) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TDE1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TDE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IHC: 1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the TDE1 serinc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TDE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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