Rabbit anti-Human DHPS Polyclonal Antibody | anti-DHPS antibody

DHPS, NT (DHPS, DS, Deoxyhypusine synthase) (APC)

Cat. Num. AAA6291971

• Gene Names: DHPS; DS; DHS; MIG13
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DHPS; Polyclonal Antibody; NT (DHPS; DS; Deoxyhypusine synthase) (APC); Deoxyhypusine synthase; anti-DHPS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-DHPS antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DHPS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human DHPS.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DHPS Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the DHPS antibody detected the DHPS protein (arrow).)

Related Product Information for anti-DHPS antibody

The unusual amino acid hypusine is formed posttranslationally and is only found in a single cellular protein, eukaryotic translation initiation factor 5A. In the first step of hypusine biosynthesis, deoxyhypusine synthase catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. This gene consists of nine exons spanning 6.6 kb. Three transcript variants have been isolated. However, only transcript variant 1 encodes an active protein. The shorter variants may act as modulating factors of DHPS activity.

Product Categories/Family for anti-DHPS antibody

Antibodies; Enzymes; Synthase

References

Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009). Alker, A.P., et al. Trop. Med. Int. Health 13(11):1384-1391(2008). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4503325
• NCBI GeneID: 1725
• NCBI Accession #: NP_001921.1; NP_037538.1
• NCBI GenBank Nucleotide #: NM_001930.3
• UniProt Accession #: P49366; Q13184; Q13276; Q9UDG0; A8K688
• Molecular Weight: 40,971 Da
• NCBI Official Full Name: deoxyhypusine synthase isoform a
• NCBI Official Synonym Full Names: deoxyhypusine synthase
• NCBI Official Symbol: DHPS
• NCBI Official Synonym Symbols: DS; DHS; MIG13
• NCBI Protein Information: deoxyhypusine synthase; migration-inducing gene 13
• UniProt Protein Name: Deoxyhypusine synthase
• UniProt Gene Name: DHPS
• UniProt Synonym Gene Names: DS; DHS
• UniProt Entry Name: DHYS_HUMAN

NCBI Description

This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2011]

Uniprot Description

DHPS: Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Belongs to the deoxyhypusine synthase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; EC 2.5.1.46; Transferase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytosol

Molecular Function: protein binding; deoxyhypusine synthase activity

Biological Process: cellular protein metabolic process; translation; positive regulation of cell proliferation; positive regulation of T cell proliferation; hypusine biosynthetic process from peptidyl-lysine; post-translational protein modification; glucose homeostasis; spermidine catabolic process to deoxyhypusine, using deoxyhypusine synthase; protein homotetramerization