Rabbit anti-Human, Mouse IGF2 Polyclonal Antibody | anti-IGF2 antibody

IGF2, ID (R54) (IGF2, Insulin-like growth factor II, Somatomedin-A, Insulin-like growth factor II Ala-25 Del, Preptin) (Azide free) (HRP)

Cat. Num. AAA6309457

• Gene Names: IGF2; GRDF; IGF-II; PP9974; C11orf43
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: IGF2; Polyclonal Antibody; ID (R54) (IGF2; Insulin-like growth factor II; Somatomedin-A; Insulin-like growth factor II Ala-25 Del; Preptin) (Azide free) (HRP); Preptin; anti-IGF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IGF2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-IGF2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 39-68 in the Central region of human IGF2. Swiss/UniProt Accession: P01344.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS6003413. This demonstrates that MBS6003413 detected the IGF2 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse cerebellum tissue lysates (35ug/lane) usingMBS6003413. This demonstrates that MBS6003413 detected the IGF2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue using MBS6003413 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6003413 for immunohistochemistry.)

Testing Data

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Product Categories/Family for anti-IGF2 antibody

Antibodies; Insulin

References

1. Adkins, R.M., et al. Pediatr. Res. 68(5):429-434(2010). 2. Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) 3. Li, J., et al. Mol. Biol. Rep. (2010) In press 4. Hsieh, Y.Y., et al. Anticancer Res. 30(6):2203-2208(2010) 5. Turan, N., et al. PLoS Genet. 6 (7), E1001033 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4504609
• NCBI GeneID: 3481
• NCBI Accession #: NP_000603
• NCBI GenBank Nucleotide #: NM_000612.5
• UniProt Accession #: P01344; P78449; Q14299; Q1WM26; Q9UC68; Q9UC69; B3KX48; B7WP08; C9JAF2; E3UN45
• Molecular Weight: 7.6 kDa (68aa), confirmed by MALDI-TOF
• NCBI Official Full Name: insulin-like growth factor II isoform 1 preproprotein
• NCBI Official Synonym Full Names: insulin like growth factor 2
• NCBI Official Symbol: IGF2
• NCBI Official Synonym Symbols: GRDF; IGF-II; PP9974; C11orf43
• NCBI Protein Information: insulin-like growth factor II
• UniProt Protein Name: Insulin-like growth factor II
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: IGF2
• UniProt Synonym Gene Names: IGF-II
• UniProt Entry Name: IGF2_HUMAN

NCBI Description

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]