Rabbit anti-Human DHFRL1 Polyclonal Antibody | anti-DHFRL1 antibody

DHFRL1, ID (DHFRL1, DHFRP4, Dihydrofolate reductase, mitochondrial, Dihydrofolate reductase-like protein 1) (FITC)

Cat. Num. AAA6291940

• Gene Names: DHFR2; DHFRL1; DHFRP4
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DHFRL1; Polyclonal Antibody; ID (DHFRL1; DHFRP4; Dihydrofolate reductase; mitochondrial; Dihydrofolate reductase-like protein 1) (FITC); Dihydrofolate reductase-like protein 1; anti-DHFRL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 187

• Applicable Applications for anti-DHFRL1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DHFRL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-74 amino acids from the Central region of human DHFRL1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DHFRL1 Antibody (Center) western blot analysis in 293,Hela,K562 cell line lysates (35ug/lane).This demonstrates the DHFRL1 antibody detected the DHFRL1 protein (arrow).)

Immunohistochemistry (IHC)

(DHFRL1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DHFRL1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-DHFRL1 antibody

DHFRL1 may play a role in folate metabolism (Potential).

Product Categories/Family for anti-DHFRL1 antibody

Antibodies; Mitochondrial Proteins

References

Semmler, A., et al. J. Neurosurg. 108(5):999-1004(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 28827793
• NCBI GeneID: 200895
• NCBI Accession #: NP_789785.1
• NCBI GenBank Nucleotide #: NP_789785.1
• UniProt Accession #: Q86XF0
• NCBI Official Full Name: dihydrofolate reductase 2, mitochondrial
• NCBI Official Synonym Full Names: dihydrofolate reductase 2
• NCBI Official Symbol: DHFR2
• NCBI Official Synonym Symbols: DHFRL1; DHFRP4
• NCBI Protein Information: dihydrofolate reductase 2, mitochondrial
• UniProt Protein Name: Dihydrofolate reductase, mitochondrial
• UniProt Gene Name: DHFRL1
• UniProt Synonym Gene Names: DHFRP4
• UniProt Entry Name: DYRL1_HUMAN

Uniprot Description

Function: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR. Ref.4 Ref.5

Catalytic activity: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH. Ref.5

Pathway: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Subcellular location: Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane Ref.4 Ref.5.

Tissue specificity: Expressed in numerous cell lines. Ref.5

Sequence similarities: Belongs to the dihydrofolate reductase family.Contains 1 DHFR (dihydrofolate reductase) domain.

Biophysicochemical propertiesKinetic parameters:KM=209 µM for dihydrofolate Ref.5KM=20 µM for NADPH