Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dihydrofolate reductase-like protein 1 (DHFRL1) Standard Curve.)
Human Dihydrofolate Reductase-Like Protein 1 (DHFRL1) ELISA Kit | DHFRL1 elisa kit
Human Dihydrofolate Reductase-Like Protein 1 (DHFRL1) ELISA Kit
Gene Names
DHFR2; DHFRL1; DHFRP4
Reactivity
Human
Synonyms
Dihydrofolate Reductase-Like Protein 1 (DHFRL1); Human Dihydrofolate Reductase-Like Protein 1 (DHFRL1) ELISA Kit; DHFRL1; DHFRP4; FLJ16119; dihydrofolate reductase pseudogene 4; DHFRL1 elisa kit
Reactivity
Human
Specificity
Human Dihydrofolate reductase-like protein 1 (DHFRL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DHFRL1. No significant cross-reactivity or interference between Human DHFRL1 and analogues was observed.
Sequence Length
187
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dihydrofolate reductase-like protein 1 (DHFRL1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dihydrofolate reductase-like protein 1 (DHFRL1) Standard Curve.)
Related Product Information for DHFRL1 elisa kit
Description: This Human Dihydrofolate reductase-like protein 1 (DHFRL1) ELISA Kit employs a two-site sandwich ELISA to quantitate DHFRL1 in samples. An antibody specific for DHFRL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DHFRL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHFRL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHFRL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR.
Background: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dihydrofolate reductase 2, mitochondrial
NCBI Official Synonym Full Names
dihydrofolate reductase 2
NCBI Official Symbol
DHFR2
NCBI Official Synonym Symbols
DHFRL1; DHFRP4
NCBI Protein Information
dihydrofolate reductase 2, mitochondrial
UniProt Protein Name
Dihydrofolate reductase, mitochondrial
UniProt Gene Name
DHFRL1
UniProt Synonym Gene Names
DHFRP4
UniProt Entry Name
DYRL1_HUMAN
Uniprot Description
Function: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR. Ref.4 Ref.5
Catalytic activity: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH. Ref.5
Pathway: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Subcellular location: Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane Ref.4 Ref.5.
Tissue specificity: Expressed in numerous cell lines. Ref.5
Sequence similarities: Belongs to the dihydrofolate reductase family.Contains 1 DHFR (dihydrofolate reductase) domain.
Biophysicochemical propertiesKinetic parameters:KM=209 µM for dihydrofolate Ref.5KM=20 µM for NADPH
Research Articles on DHFRL1
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Product Notes
The Human DHFRL1 dhfrl1 (Catalog #AAA9715731) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715731 ELISA Kit recognizes Human DHFRL1. It is sometimes possible for the material contained within the vial of "Dihydrofolate Reductase-Like Protein 1 (DHFRL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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