Western Blot (WB)
(WB Suggested Anti-DHFRL1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: THP-1 cell lysate)
Rabbit DHFR2 Polyclonal Antibody | anti-DHFR2 antibody
DHFR2 Antibody - middle region
Gene Names
DHFR2; DHFRL1; DHFRP4
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
Western Blot
Purity
Affinity Purified
Synonyms
DHFR2; Polyclonal Antibody; DHFR2 Antibody - middle region; anti-DHFR2 antibody
Host
Rabbit
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: RINLVLSRELKEPPQGAHFLARSLDDALKLTERPELANKVDMIWIVGGSS
Sequence Length
187
Applicable Applications for anti-DHFR2 antibody
Western Blot (WB)
Homology
Cow: 92%; Dog: 93%; Guinea Pig: 93%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 86%; Rat: 93%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human DHFRL1
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(WB Suggested Anti-DHFRL1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: THP-1 cell lysate)
Western Blot (WB)
(WB Suggested Anti-DHFRL1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: THP-1 cell lysate)
Related Product Information for anti-DHFR2 antibody
This is a rabbit polyclonal antibody against DHFRL1. It was validated on Western Blot using a cell lysate as a positive control.
Product Categories/Family for anti-DHFR2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21kDa
NCBI Official Full Name
dihydrofolate reductase 2, mitochondrial
NCBI Official Synonym Full Names
dihydrofolate reductase 2
NCBI Official Symbol
DHFR2
NCBI Official Synonym Symbols
DHFRL1; DHFRP4
NCBI Protein Information
dihydrofolate reductase 2, mitochondrial
UniProt Protein Name
Dihydrofolate reductase, mitochondrial
UniProt Gene Name
DHFRL1
UniProt Synonym Gene Names
DHFRP4
UniProt Entry Name
DYRL1_HUMAN
Uniprot Description
Function: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR. Ref.4 Ref.5
Catalytic activity: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH. Ref.5
Pathway: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Subcellular location: Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane Ref.4 Ref.5.
Tissue specificity: Expressed in numerous cell lines. Ref.5
Sequence similarities: Belongs to the dihydrofolate reductase family.Contains 1 DHFR (dihydrofolate reductase) domain.
Biophysicochemical propertiesKinetic parameters:KM=209 µM for dihydrofolate Ref.5KM=20 µM for NADPH
Research Articles on DHFR2
Similar Products
Product Notes
The DHFR2 dhfrl1 (Catalog #AAA3211261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHFR2 Antibody - middle region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DHFR2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the DHFR2 dhfrl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: RINLVLSREL KEPPQGAHFL ARSLDDALKL TERPELANKV DMIWIVGGSS. It is sometimes possible for the material contained within the vial of "DHFR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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