Rabbit anti-Mouse DHCR7 Polyclonal Antibody | anti-DHCR7 antibody

DHCR7, CT (DHCR7, D7SR, 7-dehydrocholesterol reductase, Putative sterol reductase SR-2, Sterol Delta(7)-reductase) (Azide free) (HRP)

Cat. Num. AAA6291930

• Gene Names: DHCR7; SLOS
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DHCR7; Polyclonal Antibody; CT (DHCR7; D7SR; 7-dehydrocholesterol reductase; Putative sterol reductase SR-2; Sterol Delta(7)-reductase) (Azide free) (HRP); Sterol Delta(7)-reductase; anti-DHCR7 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DHCR7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DHCR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-471 amino acids from the C-terminal region of human DHCR7.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DHCR7 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the DHCR7 antibody detected the DHCR7 protein (arrow).)

Immunohistochemistry (IHC)

(DHCR7 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DHCR7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of DHCR7 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-DHCR7 antibody

This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.

Product Categories/Family for anti-DHCR7 antibody

Antibodies; Enzymes; Reductase

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Koo, G., et al. Am. J. Med. Genet. A 152A (8), 2094-2098 (2010): Wang, T.J., et al. Lancet 376(9736):180-188(2010). Ahn, J., et al. Hum. Mol. Genet. 19(13):2739-2745(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 255308875
• NCBI GeneID: 1717
• NCBI Accession #: NP_001157289.1; NP_001351.2
• NCBI GenBank Nucleotide #: NM_001163817.1
• UniProt Accession #: Q9UBM7; O60492; O60717; B2R6Z2
• Molecular Weight: 54,489 Da
• NCBI Official Full Name: 7-dehydrocholesterol reductase
• NCBI Official Synonym Full Names: 7-dehydrocholesterol reductase
• NCBI Official Symbol: DHCR7
• NCBI Official Synonym Symbols: SLOS
• NCBI Protein Information: 7-dehydrocholesterol reductase
• UniProt Protein Name: 7-dehydrocholesterol reductase
• Protein Family: Probable 7-dehydrocholesterol reductase
• UniProt Gene Name: DHCR7
• UniProt Synonym Gene Names: D7SR; 7-DHC reductase

NCBI Description

This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]

Uniprot Description

Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).