Rabbit anti-Human DGKQ Polyclonal Antibody | anti-DGKQ antibody

DGKQ, CT (Diacylglycerol Kinase theta, DAG Kinase theta, Diglyceride Kinase theta, DGK-theta, DAGK4) (PE)

Cat. Num. AAA6472459

• Gene Names: DGKQ; DAGK; DAGK4; DAGK7
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: DGKQ; Polyclonal Antibody; CT (Diacylglycerol Kinase theta; DAG Kinase theta; Diglyceride Kinase theta; DGK-theta; DAGK4) (PE); anti-DGKQ antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DGKQ.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DGKQ antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human DGKQ (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DGKQ Antibody (R927) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DGKQ antibody detected the DGKQ protein (arrow).)

Immunohistochemistry (IHC)

(DGKQ Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DGKQ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DGKQ Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-DGKQ antibody

Antibodies; Protein Kinases

References

1. Pilz, A., et al., Genomics 26(3):599-601 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 40806175
• NCBI GeneID: 1609
• NCBI Accession #: NP_001338.2
• NCBI GenBank Nucleotide #: NM_001347.3
• UniProt Accession #: P52824; Q6P3W4
• Molecular Weight: 101,155 Da
• NCBI Official Full Name: diacylglycerol kinase theta
• NCBI Official Synonym Full Names: diacylglycerol kinase, theta 110kDa
• NCBI Official Symbol: DGKQ
• NCBI Official Synonym Symbols: DAGK; DAGK4; DAGK7
• NCBI Protein Information: diacylglycerol kinase theta; DGK-theta; DAG kinase theta; diglyceride kinase theta
• UniProt Protein Name: Diacylglycerol kinase theta
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: DGKQ
• UniProt Synonym Gene Names: DAGK4; DAG kinase theta; DGK-theta
• UniProt Entry Name: DGKQ_HUMAN

NCBI Description

The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKQ: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor. May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity. Belongs to the eukaryotic diacylglycerol kinase family.

Protein type: Kinase, lipid; Motility/polarity/chemotaxis; Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; EC 2.7.1.107

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: cytoskeleton; cytoplasm; plasma membrane; nuclear speck; cytosol; nucleus

Molecular Function: protein binding; phospholipase binding; transcription activator binding; metal ion binding; kinase binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; platelet activation; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; protein kinase C activation; blood coagulation; phosphorylation; response to ATP