Hyaluronidase, Bovine, Highly Purified Enzyme | HYAL3 enzyme

Hyaluronidase, Bovine, Highly Purified

Cat. Num. AAA653746

• Gene Names: HYAL3; LUCA3; HYAL-3; LUCA-3
• Purity: Molecular Biology Grade; Chromatographically purified
• Synonyms: Hyaluronidase; Bovine; Highly Purified; HYAL3 enzyme

• Host: Bovine testes
• Purity/Purification: Molecular Biology Grade; Chromatographically purified
• Form/Format: Supplied as a dialyzed, lyophilized powder

• CAS Number: 37326-33-3
• Country of Origin: The raw animal product (bovine glands, organs and tissue) used in the manufacturing of Hyaluronidase is derived from materials of USDA-approved origin. It was collected in USDA or equivalent approved facilities and inspected to be free of disease.
• Quality Control: SDS-PAGE
• Unit Definition: One unit is based on the change in absorbency (turbidity) at 540nm of an internal standard.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Prepare fresh before use..

Testing Data

(Hyaluronate 4-glycanohydrolase; Hyaluronoglucosaminidase; Hyaluronidase-1; Hyaluronoglucosaminidase-1, HYAL1, Hyal-1, EC=3.2.1.35, Hyaluronidase-2, Hyaluronoglucosaminidase-2, HYAL2, Hyal-2, EC=3.2.1.35, MGC126904)

Testing Data

(Lane 1: MW standards|Lane 2: Reference Hyaluronidase, Bovine 25mg/ml|Lane 3: Hyaluronidase, Bovine lot# L11051258 5mg/ml|Lane 4: blank|Lane 5: Hyaluronidase, Bovine lot# L11051258 10mg/ml|Lane 6: blank|Lane 7: Hyaluronidase, Bovine lot# L11051258 25mg/ml|Lane 8: blank|Lane 9: MW standards|MW standards (kD): 97.5, 66, 45, 31, 21.5, 14.4)

Testing Data

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Related Product Information for HYAL3 enzyme

Hyaluronidase catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido-2-deoxy-beta-D-glucose and D-glucose residues in hyaluronate. Hyaluronate 4-glycanohydrolase (EC 3.2.1.35) catalyzes the depolymerization of mucopolysaccharides, hyaluronic acid, and the chondroitin sulfates A and C. The enzyme is widely distributed in animal tissues but is found in great concentrations in the bovine and ovine testes. It is also produced by a number of bacteria. Hyaluronidase from bovine testes has a molecular weight of 55,000. Purified hyaluronidase is used clinically for the intradermal administration of large volumes of fluid when intravenous injections are contraindicated. The enzyme is administered prior to or simultaneously with the fluid and it facilitates absorption of the fluid.

Product Categories/Family for HYAL3 enzyme

Molecular Biology; MB-Enzymes; Glycosidase

NCBI and Uniprot Product Information

• NCBI GI #: 2935328
• NCBI GeneID: 8372
• UniProt Accession #: O43820; O60540; Q8NFK2; Q8NFK3; Q8NFK4; Q96E56; Q9BRW9
• Molecular Weight: 46,501 Da
• NCBI Official Full Name: hyaluronidase
• NCBI Official Synonym Full Names: hyaluronoglucosaminidase 3
• NCBI Official Symbol: HYAL3
• NCBI Official Synonym Symbols: LUCA3; HYAL-3; LUCA-3
• NCBI Protein Information: hyaluronidase-3; lung carcinoma protein 3
• UniProt Protein Name: Hyaluronidase-3
• UniProt Gene Name: HYAL3
• UniProt Synonym Gene Names: LUCA3; Hyal-3; LuCa-3
• UniProt Entry Name: HYAL3_HUMAN

NCBI Description

This gene encodes a member of the hyaluronidase family. Hyaluronidases are endoglycosidase enzymes that degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. The regulated turnover of hyaluronan plays a critical role in many biological processes including cell proliferation, migration and differentiation. The encoded protein may also play an important role in sperm function. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression, and the expression of specific transcript variants may be indicative of tumor status. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and some isoforms may lack hyaluronidase activity. This gene overlaps and is on the same strand as N-acetyltransferase 6 (GCN5-related), and some transcripts of each gene share a portion of the first exon. [provided by RefSeq, Jan 2011]

Uniprot Description

Catalytic activity: Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Subcellular location: Secreted

By similarity. Lysosome

By similarity.

Tissue specificity: Bone marrow, testis and kidney. Isoform 4 is detected in all bladder tumor and prostate tumor cells. Ref.1 Ref.2

Sequence similarities: Belongs to the glycosyl hydrolase 56 family.Contains 1 EGF-like domain.

Sequence caution: The sequence AAC70915.1 differs from that shown. Reason: Frameshift at position 410.