Immunofluorescence (IF)
(MBS9604665 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit DGKE Polyclonal Antibody | anti-DGKE antibody
DGKE Antibody
Gene Names
DGKE; DGK; AHUS7; DAGK5; DAGK6; NPHS7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DGKE; Polyclonal Antibody; DGKE Antibody; AHUS7; DAG kinase epsilon; DAGK 5; DAGK 6; DAGK5; DAGK6; DGK; DGK epsilon; DGK-epsilon; DGKE_HUMAN; Diacylglycerol kinase epsilon 64kDa; Diacylglycerol kinase epsilon; Diglyceride kinase epsilon; NPHS7; anti-DGKE antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DGKE antibody detects endogenous levels of total DGKE
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
567
Applicable Applications for anti-DGKE antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Tissue Specificity
Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog, Chicken
Similarity
Pig (100%), Bovine (91%), Horse (100%), Sheep (91%), Dog (100%), Chicken (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9604665 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9604665 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using DGKE antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using DGKE antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-DGKE antibody
Function: Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Similarity: Belongs to the eukaryotic diacylglycerol kinase family.
Similarity: Belongs to the eukaryotic diacylglycerol kinase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 60 kD
Predicted: 64 kDa
Predicted: 64 kDa
NCBI Official Full Name
diacylglycerol kinase epsilon
NCBI Official Synonym Full Names
diacylglycerol kinase epsilon
NCBI Official Symbol
DGKE
NCBI Official Synonym Symbols
DGK; AHUS7; DAGK5; DAGK6; NPHS7
NCBI Protein Information
diacylglycerol kinase epsilon
UniProt Protein Name
Diacylglycerol kinase epsilon
Protein Family
UniProt Gene Name
DGKE
UniProt Synonym Gene Names
DAGK5; DAG kinase epsilon; DGK-epsilon
NCBI Description
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008]
Uniprot Description
Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Research Articles on DGKE
1. mutations can lead to atypical hemolytic uremic syndrome or membranoproliferative glomerulonephritis
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Product Notes
The DGKE dgke (Catalog #AAA9604665) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DGKE Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DGKE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the DGKE dgke for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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