Western Blot (WB)
(Anti-Desmoglein 2 antibody, MBS175420, Western blottingAll lanes: Anti Desmoglein 2 (MBS175420) at 0.5ug/mlLane 1: HT1080 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugLane 4: SCG Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 122KDObserved bind size: 122KD )
Rabbit Desmoglein 2 Polyclonal Antibody | anti-DSG2 antibody
Polyclonal Anti-Desmoglein 2, DSG2
Concentration: 0.1-0.5ug/ml
Tested Species: Human, Mouse, Rat
Immunohistochemistry (IHC) Paraffin:
Concentration: 0.5-1 ug/ml
Tested Species: Human, Rat
Antigen Retrieval: By Heat
Immunocytochemistry/Immunofluorescence:
Concentration: 2ug/ml
Tested Species: Human
Flow Cytometry:
Concentration: 1-3ug/1x106 cells
Tested Species: Human
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-Desmoglein 2 antibody, MBS175420, Western blottingAll lanes: Anti Desmoglein 2 (MBS175420) at 0.5ug/mlLane 1: HT1080 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugLane 4: SCG Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 122KDObserved bind size: 122KD )
Western Blot (WB)
(Anti-Desmoglein 2 antibody, MBS175420, Western blottingAll lanes: Anti Desmoglein 2 (MBS175420) at 0.5ug/mlLane 1: HT1080 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugLane 4: SCG Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 122KDObserved bind size: 122KD )
NCBI and Uniprot Product Information
NCBI Description
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This gene product is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq, Jul 2008]
Uniprot Description
DSG2: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10); also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB). A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Protein type: Membrane protein, integral; Cell adhesion; Calcium-binding
Chromosomal Location of Human Ortholog: 18q12.1
Cellular Component: desmosome; cell surface; apical plasma membrane; integral to membrane; plasma membrane; intercellular junction; lateral plasma membrane
Molecular Function: calcium ion binding
Biological Process: maternal process involved in pregnancy; apoptosis; homophilic cell adhesion; response to progesterone stimulus; cell adhesion; cell structure disassembly during apoptosis
Disease: Cardiomyopathy, Dilated, 1bb; Arrhythmogenic Right Ventricular Dysplasia, Familial, 10
Research Articles on DSG2
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Product Notes
The DSG2 dsg2 (Catalog #AAA175420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Anti-Desmoglein 2, DSG2 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Desmoglein 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Human, Mouse, Rat Immunohistochemistry (IHC) Paraffin: Concentration: 0.5-1 ug/ml Tested Species: Human, Rat Antigen Retrieval: By Heat Immunocytochemistry/Immunofluorescence: Concentration: 2ug/ml Tested Species: Human Flow Cytometry: Concentration: 1-3ug/1x106 cells Tested Species: Human Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the DSG2 dsg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Desmoglein 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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