Rabbit Desmin Polyclonal Antibody | anti-I448 antibody

Desmin (I448) Polyclonal Antibody

Cat. Num. AAA3001563

• Gene Names: DES; CSM1; CSM2; CDCD3; CMD1F; LGMD1D; LGMD1E; LGMD2R
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Desmin; Polyclonal Antibody; Desmin (I448) Polyclonal Antibody; DES; anti-I448 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Desmin (I448) polyclonal antibody detects endogenous levels of Desmin protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 470

• Applicable Applications for anti-I448 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 420-470 of Human Desmin.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Desmin (I448) pAb in paraffin-embedded human skeletal muscle tissue.)

Related Product Information for anti-I448 antibody

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family such as the cytokeratins, in neoplasms including melanoma and breast carcinoma. Vimentin and Desmin, a related class III intermediate filament, are both expressed during skeletal muscle development.

NCBI and Uniprot Product Information

• NCBI GI #: 55749932
• NCBI GeneID: 1674
• NCBI Accession #: NP_001918.3
• NCBI GenBank Nucleotide #: NP_001918.3
• UniProt Accession #: P17661
• Molecular Weight: ~ 54kDa
• NCBI Official Full Name: desmin
• NCBI Official Synonym Full Names: desmin
• NCBI Official Symbol: DES
• NCBI Official Synonym Symbols: CSM1; CSM2; CDCD3; CMD1F; LGMD1D; LGMD1E; LGMD2R
• NCBI Protein Information: desmin
• UniProt Protein Name: Desmin
• Protein Family: Desmin
• UniProt Gene Name: DES
• UniProt Entry Name: DESM_HUMAN

NCBI Description

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]

Uniprot Description

desmin: a class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: intermediate filament; fascia adherens; Z disc; cytosol; neuromuscular junction; sarcolemma

Molecular Function: identical protein binding; protein binding; structural constituent of cytoskeleton; cytoskeletal protein binding

Biological Process: muscle contraction; cytoskeleton organization and biogenesis; regulation of heart contraction; muscle filament sliding

Disease: Cardiomyopathy, Dilated, 1i; Scapuloperoneal Syndrome, Neurogenic, Kaeser Type; Myopathy, Myofibrillar, 1; Muscular Dystrophy, Limb-girdle, Type 2r