Western Blot (WB)
(Anti-DEPTOR Antibody (N-Term) at 1:2000 dilution + RPMI 8226 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human DEPTOR Polyclonal Antibody | anti-DEPTOR antibody
DEPTOR Antibody (N-Term)
Gene Names
DEPTOR; DEP.6; DEPDC6
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
DEPTOR; Polyclonal Antibody; DEPTOR Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); DEP domain-containing mTOR-interacting protein; DEP domain-containing protein 6; DEPDC6; anti-DEPTOR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This DEPTOR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3-38 amino acids from the human region of human DEPTOR.
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
308
Applicable Applications for anti-DEPTOR antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:2000
Antigen Type
Synthesized Peptide
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-DEPTOR Antibody (N-Term) at 1:2000 dilution + RPMI 8226 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-DEPTOR Antibody (N-Term) at 1:2000 dilution + RPMI 8226 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-DEPTOR antibody
Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.
Product Categories/Family for anti-DEPTOR antibody
References
Wiemann S.,et al.Genome Res. 11:422-435(2001). Ota T.,et al.Nat. Genet. 36:40-45(2004). Nusbaum C.,et al.Nature 439:331-335(2006). Mural R.J.,et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46294
NCBI Official Full Name
DEP domain-containing mTOR-interacting protein isoform 2
NCBI Official Synonym Full Names
DEP domain containing MTOR interacting protein
NCBI Official Symbol
DEPTOR
NCBI Official Synonym Symbols
DEP.6; DEPDC6
NCBI Protein Information
DEP domain-containing mTOR-interacting protein
UniProt Protein Name
DEP domain-containing mTOR-interacting protein
Protein Family
UniProt Gene Name
DEPTOR
UniProt Synonym Gene Names
DEPDC6
Uniprot Description
Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.
Research Articles on DEPTOR
Similar Products
Product Notes
The DEPTOR deptor (Catalog #AAA9231232) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DEPTOR Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DEPTOR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:2000. Researchers should empirically determine the suitability of the DEPTOR deptor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEPTOR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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