Human Interleukin 24 ELISA Kit | Il 24 elisa kit

Human Interleukin 24 ELISA Kit

Cat. Num. AAA740963

• Gene Names: IL24; C49A; FISP; MDA7; MOB5; ST16; IL10B
• Reactivity: Human
• Synonyms: Interleukin 24; Human Interleukin 24 ELISA Kit; Il 24 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This IL24 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IL24. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Il 24 elisa kit

Principle of the Assay: IL24 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL24 Standards or samples are then added to the microtiter plate wells and IL24 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL24 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for IL24 are added to each well to "sandwich" the IL24 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL24 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL24 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Il 24 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 16307185
• NCBI GeneID: 11009
• Molecular Weight: 7,347 Da
• NCBI Official Full Name: Interleukin 24
• NCBI Official Synonym Full Names: interleukin 24
• NCBI Official Symbol: IL24
• NCBI Official Synonym Symbols: C49A; FISP; MDA7; MOB5; ST16; IL10B
• NCBI Protein Information: interleukin-24; melanocyte-associated Mda-7; IL-4-induced secreted protein; melanoma differentiation-associated gene 7 protein; suppression of tumorigenicity 16 (melanoma differentiation)
• UniProt Protein Name: Interleukin-24
• UniProt Gene Name: IL24
• UniProt Synonym Gene Names: MDA7; ST16; IL-24; MDA-7
• UniProt Entry Name: IL24_HUMAN

NCBI Description

This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL24: Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. Belongs to the IL-10 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; endoplasmic reticulum

Molecular Function: cytokine activity

Biological Process: negative regulation of cell proliferation; wound healing; apoptosis; positive regulation of cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of caspase activity; immune response; negative regulation of caspase activity; inflammatory response; negative regulation of cell migration; serine phosphorylation of STAT3 protein; positive regulation of tyrosine phosphorylation of Stat3 protein