Rabbit anti-Human, Rat DENND1C Polyclonal Antibody | anti-DENND1C antibody

DENND1C Antibody

Cat. Num. AAA7052985

• Gene Names: DENND1C; FAM31C
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: DENND1C; Polyclonal Antibody; DENND1C Antibody; DENN domain-containing protein 1C; Connecdenn 3; FAM31C; anti-DENND1C antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 757

• Applicable Applications for anti-DENND1C antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200
• Immunogen: Recombinant Human DENN domain-containing protein 1C protein (601-801AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Rat liver tissue,Rat lung tissueAll lanes: DENND1C antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 88,83 KDaObserved band size: 88 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lymph node tissue using MBS7052985 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung tissue using MBS7052985 at dilution of 1:100)

Related Product Information for anti-DENND1C antibody

Guanine nucleotide exchange factor (GEF) which may activate RAB8A, RAB13 and RAB35. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.

NCBI and Uniprot Product Information

• NCBI GI #: 594140615
• NCBI GeneID: 79958
• NCBI Accession #: NP_001277260.1
• NCBI GenBank Nucleotide #: NM_001290331.1
• UniProt Accession #: Q8IV53; Q8NDB1; Q9H5Z2; B4E051; D3PFD4
• Molecular Weight: 82,126 Da
• NCBI Official Full Name: DENN domain-containing protein 1C isoform 2
• NCBI Official Synonym Full Names: DENN domain containing 1C
• NCBI Official Symbol: DENND1C
• NCBI Official Synonym Symbols: FAM31C
• NCBI Protein Information: DENN domain-containing protein 1C
• UniProt Protein Name: DENN domain-containing protein 1C
• Protein Family: DENN domain-containing protein
• UniProt Gene Name: DENND1C
• UniProt Synonym Gene Names: FAM31C

NCBI Description

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1C, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]

Uniprot Description

DENND1C: a guanine nucleotide exchange factor (GEF) associated with clathrin-associated vesicles. Appears to activate RAB35, RAB8A, and RAB13. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Exhibits low nucleotide-independent RAB35-binding activity. Interacts with clathrin heavy chain/CLTC and with AP2A2, but not with AP2B1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Rab

Chromosomal Location of Human Ortholog: 19p13.3

Molecular Function: Rab guanyl-nucleotide exchange factor activity