Rabbit MAPK1 Polyclonal Antibody | anti-MAPK1 antibody

MAPK1 antibody

Cat. Num. AAA5300794

• Gene Names: MAPK1; ERK2
• Applications: Western Blot
• Synonyms: MAPK1; Polyclonal Antibody; MAPK1 antibody; Polyclonal MAPK1; Anti-MAPK1; MAPK 1; mitogen-activated protein kinase 1; p38; PRKM2; P42MAPK; PRKM1; p41; p40; MAPK2; ERK; ERK2; MAPK-1; ERT1; p41mapk; anti-MAPK1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Form/Format: Lyophilized powder. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 360

• Applicable Applications for anti-MAPK1 antibody: Western Blot (WB)
• Application Notes: WB: 0.2-1 ug/ml
• Biological Significance: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
• Cross-Reactivity: Human
• Immunogen: MAPK1 antibody was raised in rabbit using the middle region of MAPK1 as the immunogen
• Preparation and Storage: Store at 4 degree C, following reconstitution, aliquot and store at -20 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Recommended MAPK1 Antibody Titration: 0.2-1 ug/ml)

Western Blot (WB)

(Recommended MAPK1 Antibody Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug U205 lysate; Antibody Dilution: 1:1000)

Related Product Information for anti-MAPK1 antibody

Affinity purified rabbit polyclonal MAPK1 antibody

Product Categories/Family for anti-MAPK1 antibody

Protein Modification & Stress Response; Purified Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 28461209
• NCBI GeneID: 327672
• NCBI Accession #: NP_786987.1
• NCBI GenBank Nucleotide #: NM_175793.2
• Molecular Weight: 41,376 Da
• NCBI Official Full Name: mitogen-activated protein kinase 1
• NCBI Official Symbol: MAPK1
• NCBI Official Synonym Symbols: ERK2
• NCBI Protein Information: mitogen-activated protein kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase 1
• UniProt Gene Name: MAPK1
• UniProt Synonym Gene Names: ERK2; PRKM1; MAP kinase 1; MAPK 1; ERK-2; MAP kinase 2; MAPK 2
• UniProt Entry Name: MK01_BOVIN

Uniprot Description

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. May play a role in the spindle assembly checkpoint (). Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation ().