Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5% SDS PAGE gel and DENND1B antibody at a dilution of 1:1000)
Rabbit DENND1B Polyclonal Antibody | anti-DENND1B antibody
DENND1B Antibody
Gene Names
DENND1B; FAM31B; C1ORF18; C1orf218
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
DENND1B; Polyclonal Antibody; DENND1B Antibody; Affinity Purified Rabbit Polyclonal DENND1B Antibody; Polyclonal DENND1B antibody; Anti-DENND1B antibody; MGC103810 antibody; C1ORF18 antibody; FLJ33888 antibody; FLJ20054 antibody; DENNDB-1; DENNDB 1; DENND1B antibody; MGC27044 antibody; DENNDB-1 antibody; FLJ21179 antibody; FAM31B antibody; family with sequence similarity 31 member B antibody; DKFZp547O0715 antibody; DENNDB 1 antibody; C1orf218 antibody; anti-DENND1B antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.9 mg/ml (varies by lot)
Sequence Length
396
Applicable Applications for anti-DENND1B antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
WB: 1:500-1:3000
ICC: 1:100-1:500
WB: 1:500-1:3000
Immunogen
DENND1B antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 232 of DENND1B
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5% SDS PAGE gel and DENND1B antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5% SDS PAGE gel and DENND1B antibody at a dilution of 1:1000)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DENND1B antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DENND1B antibody at 1:200 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: MOLT4) using a 7.5% SDS PAGE gel and DENND1B antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: MOLT4) using a 7.5% SDS PAGE gel and DENND1B antibody at a dilution of 1:1000)
Related Product Information for anti-DENND1B antibody
Clathrin-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors for the early endosomal small GTPase RAB35 and bind to clathrin and clathrin adaptor protein-2. Thus, connecdenns link RAB35 activation with the clathrin machinery.
Product Categories/Family for anti-DENND1B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48 kDa
NCBI Official Full Name
DENN domain-containing protein 1B isoform 5
NCBI Official Synonym Full Names
DENN domain containing 1B
NCBI Official Symbol
DENND1B
NCBI Official Synonym Symbols
FAM31B; C1ORF18; C1orf218
NCBI Protein Information
DENN domain-containing protein 1B
UniProt Protein Name
DENN domain-containing protein 1B
Protein Family
UniProt Gene Name
DENND1B
UniProt Synonym Gene Names
C1orf218; FAM31B
UniProt Entry Name
DEN1B_HUMAN
NCBI Description
Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]
Research Articles on DENND1B
Similar Products
Product Notes
The DENND1B dennd1b (Catalog #AAA5312133) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DENND1B can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). IF: 1:100-1:500 ICC: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the DENND1B dennd1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DENND1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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