Rabbit anti-Human DENND1A Polyclonal Antibody | anti-DENND1A antibody

DENND1A Polyclonal Antibody

Cat. Num. AAA9131133

• Gene Names: DENND1A; FAM31A; KIAA1608
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: DENND1A; Polyclonal Antibody; DENND1A Polyclonal Antibody; FAM31A; KIAA1608; anti-DENND1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 1009

• Applicable Applications for anti-DENND1A antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:2000
• Immunogen: Recombinant protein of human DENND1A
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Related Product Information for anti-DENND1A antibody

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1A, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).

Product Categories/Family for anti-DENND1A antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 109825594
• NCBI GeneID: 57706
• UniProt Accession #: Q8TEH3; Q05C88; Q5VWF0; Q6PJZ5; Q8IVD6; Q9H796; A8MZA3; B1AM80; B7Z3C8; B7Z669; D3PFD3
• Molecular Weight: 111kDa
• NCBI Official Full Name: DENN domain-containing protein 1A
• NCBI Official Synonym Full Names: DENN domain containing 1A
• NCBI Official Symbol: DENND1A
• NCBI Official Synonym Symbols: FAM31A; KIAA1608
• NCBI Protein Information: DENN domain-containing protein 1A
• UniProt Protein Name: DENN domain-containing protein 1A
• Protein Family: DENN domain-containing protein
• UniProt Gene Name: DENND1A
• UniProt Synonym Gene Names: Connecdenn

NCBI Description

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1A, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]

Uniprot Description

DENND1A: a guanine nucleotide exchange factor (GEF) associated with clathrin-coated vesicle and presynaptic membranes. Regulates clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles. Interacts with ITSN1 AND SH3GL2. Interacts with RAB35. Interacts with clathrin and with the adapter protein complex 2, AP-2. Localizes to clathrin patches at the plasma membrane and activatesRab35 in an endocytic pathway trafficking Shiga toxin to the trans-Golgi network. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: GEF; GEF, Rab; Vesicle

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: clathrin coated vesicle membrane; clathrin-coated vesicle; cytosol; intracellular membrane-bound organelle; nucleoplasm

Molecular Function: phosphatidylinositol 3-phosphate binding; Rab guanyl-nucleotide exchange factor activity

Biological Process: endocytic recycling; endocytosis; regulation of Rab protein signal transduction