Rabbit anti-Human DENND1A Polyclonal Antibody | anti-DENND1A antibody

DENND1A Antibody

Cat. Num. AAA1492989

• Gene Names: DENND1A; FAM31A; KIAA1608
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: >95%, Protein G Purified
• Synonyms: DENND1A; Polyclonal Antibody; DENND1A Antibody; DENN domain-containing protein 1A; FAM31A; KIAA1608; Connecdenn 1; Connecdenn; Protein FAM31A; anti-DENND1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G Purified
• Sequence Length: 1009

• Applicable Applications for anti-DENND1A antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: IHC:1:20-1:200
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant human DENN domain-containing protein 1A protein
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS1492989 at dilution 1:100)

Related Product Information for anti-DENND1A antibody

Guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles.

NCBI and Uniprot Product Information

• NCBI GI #: 55749779
• NCBI GeneID: 57706
• NCBI Accession #: NP_065997.1
• NCBI GenBank Nucleotide #: NM_020946.1
• UniProt Accession #: Q8TEH3; Q05C88; Q5VWF0; Q6PJZ5; Q8IVD6; Q9H796; A8MZA3; B1AM80; B7Z3C8; B7Z669; D3PFD3
• Molecular Weight: 111,005 Da
• NCBI Official Full Name: DENN domain-containing protein 1A isoform 1
• NCBI Official Synonym Full Names: DENN/MADD domain containing 1A
• NCBI Official Symbol: DENND1A
• NCBI Official Synonym Symbols: FAM31A; KIAA1608
• NCBI Protein Information: DENN domain-containing protein 1A
• UniProt Protein Name: DENN domain-containing protein 1A
• Protein Family: DENN domain-containing protein
• UniProt Gene Name: DENND1A
• UniProt Synonym Gene Names: FAM31A; KIAA1608; Connecdenn
• UniProt Entry Name: DEN1A_HUMAN

NCBI Description

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1A, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]

Uniprot Description

DENND1A: a guanine nucleotide exchange factor (GEF) associated with clathrin-coated vesicle and presynaptic membranes. Regulates clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles. Interacts with ITSN1 AND SH3GL2. Interacts with RAB35. Interacts with clathrin and with the adapter protein complex 2, AP-2. Localizes to clathrin patches at the plasma membrane and activatesRab35 in an endocytic pathway trafficking Shiga toxin to the trans-Golgi network. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rab; GEFs; Vesicle

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: cell junction; cell soma; clathrin coated vesicle membrane; clathrin-coated vesicle; dendrite; presynaptic membrane

Molecular Function: Rab guanyl-nucleotide exchange factor activity; SH3 domain binding

Biological Process: endocytosis; positive regulation of GTPase activity; protein transport; regulation of Rab protein signal transduction; synaptic vesicle endocytosis