Immunohistochemistry (IHC)
((0.1ug/ml) staining of nuclear HeLa (A) and A431 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
FBXO11 Antibody | anti-FBXO11 antibody
Goat anti-FBXO11 (aa754-765) Antibody
Expected from sequence similarity: Mouse, Rat, Pig, Cow
Expected from sequence similarity: Mouse, Rat, Pig, Cow
WB: Approx 90kDa band observed in nuclear lysates of cell line HeLa and lysates of cell line A431. Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.
Immunohistochemistry (IHC)
((0.1ug/ml) staining of nuclear HeLa (A) and A431 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((0.1ug/ml) staining of nuclear HeLa (A) and A431 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2010]
Uniprot Description
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as DTL/CDT2, BCL6 and PRDM1/BLIMP1. The SCF(FBXO11) complex mediates ubiquitination and degradation of BCL6, thereby playing a role in the germinal center B-cells terminal differentiation toward memory B-cells and plasma cells. The SCF(FBXO11) complex also mediates ubiquitination and degradation of DTL, an important step for the regulation of TGF-beta signaling, cell migration and the timing of the cell-cycle progression and exit. Binds to and neddylates phosphorylated p53/TP53, inhibiting its transcriptional activity. SCF(FBXO11) does not seem to direct ubiquitination of p53/TP53.
Research Articles on FBXO11
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Product Notes
The FBXO11 fbxo11 (Catalog #AAA423786) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FBXO11 (aa754-765) Antibody reacts with Human Expected from sequence similarity: Mouse, Rat, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's FBXO11 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Pep-ELISA: antibody detection limit dilution 1:16000. WB: Approx 90kDa band observed in nuclear lysates of cell line HeLa and lysates of cell line A431. Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. Researchers should empirically determine the suitability of the FBXO11 fbxo11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FBXO11, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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