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Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Rabbit DEM1 Polyclonal Antibody | anti-DEM1 antibody
DEM1, ID (DEM1, C1orf176, Probable exonuclease V, Defects in morphology protein 1 homolog) (Biotin)
Gene Names
EXO5; DEM1; Exo V; hExo5; C1orf176
Reactivity
Human, Hamster, Mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DEM1; Polyclonal Antibody; ID (DEM1; C1orf176; Probable exonuclease V; Defects in morphology protein 1 homolog) (Biotin); Defects in morphology protein 1 homolog; anti-DEM1 antibody
Host
Rabbit
Reactivity
Human, Hamster, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-DEM1 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
DEM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 173-201 amino acids from the Central region of human DEM1.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Flow Cytometry (FC/FACS)
(DEM1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DEM1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-DEM1 antibody
References
Lamesch, P., et al. Genomics 89(3):307-315(2007). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,816 Da
NCBI Official Full Name
exonuclease V
NCBI Official Synonym Full Names
exonuclease 5
NCBI Official Symbol
EXO5
NCBI Official Synonym Symbols
DEM1; Exo V; hExo5; C1orf176
NCBI Protein Information
exonuclease V; probable exonuclease V; defects in morphology 1 homolog; defects in morphology protein 1 homolog
UniProt Protein Name
Exonuclease V
UniProt Gene Name
EXO5
UniProt Synonym Gene Names
C1orf176; DEM1; Exo V; hExo5
Uniprot Description
DEM1: Probable single strand DNA specific 5' exonuclease mitochondrial DNA replication and recombination. Belongs to the EXO5 family.
Protein type: EC 3.1.11.-
Chromosomal Location of Human Ortholog: 1p34.2
Cellular Component: cytosol; nucleoplasm; nucleus
Molecular Function: 4 iron, 4 sulfur cluster binding; DNA binding; metal ion binding; protein homodimerization activity; single-stranded DNA specific 3'-5'exodeoxyribonuclease activity; single-stranded DNA specific 5'-3'exodeoxyribonuclease activity
Biological Process: nucleic acid phosphodiester bond hydrolysis
Similar Products
Product Notes
The DEM1 exo5 (Catalog #AAA6291675) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DEM1, ID (DEM1, C1orf176, Probable exonuclease V, Defects in morphology protein 1 homolog) (Biotin) reacts with Human, Hamster, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DEM1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DEM1 exo5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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