Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Rabbit DEM1 Polyclonal Antibody | anti-EXO5 antibody
DEM1, ID (DEM1, C1orf176, Probable exonuclease V, Defects in morphology protein 1 homolog)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Western Blot (WB)
(DEM1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).)
Flow Cytometry (FC/FACS)
(DEM1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DEM1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity. Ref.5
Cofactor: Binds 1 4Fe-4S cluster. Ref.5Magnesium
By similarity. Ref.5
Subunit structure: Monomer; monomeric form has weak exonuclease activity. Homodimer; homodimeric form is unsure but has much higher exonuclease activity, suggesting that it could homodimerize upon DNA-binding. Interacts with the replication protein A (RPA) complex. Ref.5
Subcellular location: Nucleus. Cytoplasm › cytosol. Note: Localizes to repair foci in response to DNA damage. Ref.5
Sequence similarities: Belongs to the EXO5 family.
Sequence caution: The sequence CAI16298.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI16299.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI16300.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI16301.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI16302.1 differs from that shown. Reason: Erroneous gene model prediction.
Research Articles on EXO5
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Product Notes
The EXO5 exo5 (Catalog #AAA648535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DEM1, ID (DEM1, C1orf176, Probable exonuclease V, Defects in morphology protein 1 homolog) reacts with Hamster, Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DEM1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the EXO5 exo5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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