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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Rat Syndecan 4 (SDC4) Polyclonal Antibody | anti-SDC4 antibody

Polyclonal Antibody to Syndecan 4 (SDC4)

Gene Names
Sdc4; RYUDOCA; RATRYUDOCA
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Syndecan 4 (SDC4); Polyclonal Antibody; Polyclonal Antibody to Syndecan 4 (SDC4); anti-SDC4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SDC4. It has been selected for its ability to recognize SDC4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGSEF-GESIRETE VIDPQDLLEG RYFSGALPDD EDAGGLEQDSDFELSGSGDL DDTEEPRTFP EVISPLVPLD NHIPENAQPG IRVPSEPKEL EENEVIPKRV PSDVGDDDVS NKVSMSSTSQ GSNIFERTE
Sequence Length
198
Applicable Applications for anti-SDC4 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant SDC4 (Gly23~Glu149) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2060832
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded pancreas tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded pancreas tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,962 Da
NCBI Official Full Name
syndecan-4
NCBI Official Synonym Full Names
syndecan 4
NCBI Official Symbol
Sdc4
NCBI Official Synonym Symbols
RYUDOCA; RATRYUDOCA
NCBI Protein Information
syndecan-4
UniProt Protein Name
Syndecan-4
Protein Family
UniProt Gene Name
Sdc4
UniProt Synonym Gene Names
Synd4; SYND4

NCBI Description

regulates cell-matrix interactions; may bind phospholipids and play a role in phosphoinositide-mediated signaling [RGD, Feb 2006]

Uniprot Description

syndecan-4: a heparan sulfate proteoglycan type I membrane protein that belongs to the syndecan proteoglycan family. Expressed as a homodimer. Promotes cell spreading in a beta(1) integrin-dependent fashion through PKC-alpha and Rho.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q42

Cellular Component: cell surface; costamere; extracellular exosome; focal adhesion; integral component of membrane; membrane raft; plasma membrane

Molecular Function: fibronectin binding; identical protein binding; protein binding; protein kinase C binding

Biological Process: cell adhesion; cell migration; cell-cell signaling; inner ear receptor stereocilium organization and biogenesis; negative regulation of T cell proliferation; neural tube closure; positive regulation of focal adhesion assembly; positive regulation of protein kinase activity; positive regulation of stress fiber formation; regulation of aortic smooth muscle cell differentiation; regulation of fibroblast migration; signal transduction; ureteric bud development; wound healing

Research Articles on SDC4

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Product Notes

The SDC4 sdc4 (Catalog #AAA2006021) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Syndecan 4 (SDC4) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Syndecan 4 (SDC4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SDC4 sdc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-GESIRE TE VIDPQDLLEG RYFSGALPDD EDAGGLEQDS DFELSGSGDL DDTEEPRTFP EVISPLVPLD NHIPENAQPG IRVPSEPKEL EENEVIPKRV PSDVGDDDVS NKVSMSSTSQ GSNIFERTE. It is sometimes possible for the material contained within the vial of "Syndecan 4 (SDC4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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