Rabbit anti-Human, Mouse Defender of Apoptotic Cell Death 1 Polyclonal Antibody | anti-DAD1 antibody

Defender of Apoptotic Cell Death 1 (DAD1) (APC)

Cat. Num. AAA6472164

• Gene Names: DAD1; OST2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: Defender of Apoptotic Cell Death 1; Polyclonal Antibody; Defender of Apoptotic Cell Death 1 (DAD1) (APC); anti-DAD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Specific for defender of apoptotic cell death 1 (DAD1). Species Crossreactivity: Mouse
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-DAD1 antibody: Western Blot (WB)
• Application Notes: WB: 0.5-2ug/ml, detects a band of approximately 22kD in HepG2 cell lysates.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a 14aa sequence within the C-Terminal region of human DAD1.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-DAD1 antibody

DAD1 was discovered as a negative regulator of programmed cell death, and has since been identified as a subunit of the mammalian oligosaccharyltransferase complex (OCT). DAD1 is required for the function and structural integrity of OCT.

Product Categories/Family for anti-DAD1 antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 14602573
• NCBI GeneID: 1603
• UniProt Accession #: P61803; O08552; O70364; P46966; P46968; Q6FGA3; Q96GB7; D3DS25
• Molecular Weight: 12,497 Da
• NCBI Official Full Name: Defender against cell death 1
• NCBI Official Synonym Full Names: defender against cell death 1
• NCBI Official Symbol: DAD1
• NCBI Official Synonym Symbols: OST2
• NCBI Protein Information: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; DAD-1; oligosaccharyltransferase 2 homolog; oligosaccharyl transferase subunit DAD1; oligosaccharyltransferase subunit 2 (non-catalytic)
• UniProt Protein Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
• Protein Family: DASH complex
• UniProt Gene Name: DAD1
• UniProt Synonym Gene Names: Oligosaccharyl transferase subunit DAD1; DAD-1
• UniProt Entry Name: DAD1_HUMAN

NCBI Description

DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]

Uniprot Description

DAD1: Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis. Belongs to the DAD/OST2 family.

Protein type: Endoplasmic reticulum; Transferase; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Apoptosis; Membrane protein, multi-pass; EC 2.4.99.18

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: endoplasmic reticulum membrane; membrane; oligosaccharyl transferase complex; integral to membrane

Molecular Function: dolichyl-diphosphooligosaccharide-protein glycotransferase activity; oligosaccharyl transferase activity

Biological Process: response to drug; blastocyst development; cellular protein metabolic process; apoptosis; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; response to nutrient; negative regulation of apoptosis