Mouse Neurofilament Monoclonal Antibody | anti-NF antibody

Neurofilament (NF 200kD, NF-200, NF-H, NF) (Biotin)

Cat. Num. AAA6122371

• Gene Names: NEFH; NFH
• Reactivity: Chicken, Human, Mouse, Porcine, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Neurofilament; Monoclonal Antibody; Neurofilament (NF 200kD; NF-200; NF-H; NF) (Biotin); anti-NF antibody

• Host: Mouse
• Reactivity: Chicken, Human, Mouse, Porcine, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: RT97
• Specificity: Recognizes rat neurofilament. Species Crossreactivity: human, mouse, chicken and porcine.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.

• Applicable Applications for anti-NF antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin
• Application Notes: IHC-P: Staining of formalin-fixed tissues requires boiling tissues sections in 10mM sodium citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.; Applications are based on unconjugated antibody.
• Immunogen: Triton-X 100 insoluble protein fraction of rat brain
• Conjugate: Biotin
• Positive Control: Brain, Neuroblastoma
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human cerebellum stained with (1:200), peroxidase-conjugate and DAB chromogen.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human cerebellum stained with (1:200), peroxidase-conjugate and DAB chromogen.)

Product Categories/Family for anti-NF antibody

Antibodies; Neurofilament

References

1. Ishii M et. al. Fukushima J Med Sci. 2004;50(2):65-74.

NCBI and Uniprot Product Information

• NCBI GI #: 32483416
• NCBI GeneID: 4744
• NCBI Accession #: NP_066554
• NCBI GenBank Nucleotide #: NM_021076
• UniProt Accession #: P12036; P16884
• Molecular Weight: 105,639 Da
• NCBI Official Full Name: neurofilament heavy polypeptide
• NCBI Official Synonym Full Names: neurofilament, heavy polypeptide
• NCBI Official Symbol: NEFH
• NCBI Official Synonym Symbols: NFH
• NCBI Protein Information: neurofilament heavy polypeptide; 200 kDa neurofilament protein; NF-H; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa
• UniProt Protein Name: Neurofilament heavy polypeptide
• Protein Family: Neurofilament heavy polypeptide
• UniProt Gene Name: NEFH
• UniProt Synonym Gene Names: KIAA0845; NFH; NF-H
• UniProt Entry Name: NFH_HUMAN

Uniprot Description

NFH: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: mitochondrion; axon; cytoplasm; neurofilament

Molecular Function: protein binding, bridging; dynein binding; structural constituent of cytoskeleton; kinesin binding; microtubule binding; structural molecule activity; protein kinase binding

Biological Process: axonogenesis; neurofilament bundle assembly; microtubule cytoskeleton organization and biogenesis; cell projection biogenesis; peripheral nervous system neuron axonogenesis

Disease: Amyotrophic Lateral Sclerosis 1