Goat DEFA1 / HP1 Polyclonal Antibody | anti-DEFA1 antibody

Goat Polyclonal to Human DEFA1 / HP1

Cat. Num. AAA246828

• Gene Names: DEFA1; HP1; MRS; DEF1; HP-1; DEFA2; HNP-1
• Reactivity: Chimpanzee, Gorilla, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: DEFA1 / HP1; Polyclonal Antibody; Goat Polyclonal to Human DEFA1 / HP1; Anti-DEFA1 / HP1 Antibody (Internal) IHC-plus; DEFA1; DEFA2; Defensin; alpha 2; HP-1; HP1; Myeloid-related sequence; MRS; DEF1; alpha 1; HNP-1; Neutrophil defensin 1; Human DEFA1; anti-DEFA1 antibody

• Host: Goat
• Reactivity: Chimpanzee, Gorilla, Human
• Clonality: Polyclonal
• Specificity: Human DEFA1. This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 94

• Applicable Applications for anti-DEFA1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (3.75 ug/ml), WB (1 - 3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 10kDa band observed in Human Spleen lysates (calculated MW of 10.2kDa according to NP_004075.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown ident...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-DESLAPKHPGSRKN from an internal region of human DEFA1 (NP_004075.1; NP_005208.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon (86%).
• Immunogen Type: Synthetic peptide
• Immunogen: DEFA1 / HP1 antibody was raised against synthetic peptide C-DESLAPKHPGSRKN from an internal region of human DEFA1 (NP_004075.1; NP_005208.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon (86%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-DEFA1 / NP-1 antibody IHC of human thymus, myeloid cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(DEFA1 antibody (2 ug/ml) staining of Human Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-DEFA1 antibody

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes appear to be clustered on chromosome 8.

NCBI and Uniprot Product Information

• NCBI GI #: 4758146
• NCBI GeneID: 1667
• NCBI Accession #: NP_004075.1
• NCBI GenBank Nucleotide #: NM_004084.3
• UniProt Accession #: P59665; P11479; Q14125; Q6EZF6
• Molecular Weight: 10,201 Da
• NCBI Official Full Name: neutrophil defensin 1 preproprotein
• NCBI Official Synonym Full Names: defensin, alpha 1
• NCBI Official Symbol: DEFA1
• NCBI Official Synonym Symbols: HP1; MRS; DEF1; HP-1; DEFA2; HNP-1
• NCBI Protein Information: neutrophil defensin 1; defensin, alpha 2; myeloid-related sequence; defensin, alpha 1, myeloid-related sequence
• UniProt Protein Name: Neutrophil defensin 1
• Protein Family: Alpha-defensin
• UniProt Gene Name: DEFA1
• UniProt Synonym Gene Names: DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2
• UniProt Entry Name: DEF1_HUMAN

NCBI Description

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, Oct 2014]

Uniprot Description

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to fungus; defense response to virus