Rabbit anti-Human, Mouse DEF6 Polyclonal Antibody | anti-DEF6 antibody

DEF6 Antibody

Cat. Num. AAA7051404

• Gene Names: DEF6; IBP; SLAT; SWAP70L
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: DEF6; Polyclonal Antibody; DEF6 Antibody; Differentially expressed in FDCP 6 homolog; DEF-6; IRF4-binding protein; IBP; anti-DEF6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 631

• Applicable Applications for anti-DEF6 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Differentially expressed in FDCP 6 homolog protein (20-114AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Mouse liver tissue,Mouse kidney tissueAll lanes: DEF6 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 74 kDaObserved band size: 74 kDa)

Immunofluorescence (IF)

(Immunofluorescent analysis of U251 cells using MBS7051404 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-DEF6 antibody

Phosphatidylinositol 3, 4, 5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 145312241
• NCBI GeneID: 50619
• NCBI Accession #: NP_071330.3
• NCBI GenBank Nucleotide #: NM_022047.3
• UniProt Accession #: Q9H4E7; Q86VF4
• Molecular Weight: 73,910 Da
• NCBI Official Full Name: differentially expressed in FDCP 6 homolog
• NCBI Official Synonym Full Names: DEF6, guanine nucleotide exchange factor
• NCBI Official Symbol: DEF6
• NCBI Official Synonym Symbols: IBP; SLAT; SWAP70L
• NCBI Protein Information: differentially expressed in FDCP 6 homolog
• UniProt Protein Name: Differentially expressed in FDCP 6 homolog
• Protein Family: Differentially expressed in FDCP
• UniProt Gene Name: DEF6
• UniProt Synonym Gene Names: IBP; DEF-6
• UniProt Entry Name: DEFI6_HUMAN

NCBI Description

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).[supplied by OMIM, Mar 2008]

Uniprot Description

DEF6: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling.

Protein type: GEFs; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 6p21.33-p21.1

Cellular Component: membrane

Molecular Function: protein binding