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Western Blot (WB)
(DEF Antibody (N-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DEF antibody detected the DEF protein (arrow).)
Rabbit DEF Polyclonal Antibody | anti-DIEXF antibody
DEF Antibody (N-term)
Gene Names
DIEXF; DEF; UTP25; C1orf107; DJ434O14.5
Reactivity
Mouse, hamster (Predicted Reactivity: Rat)
Applications
Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DEF; Polyclonal Antibody; DEF Antibody (N-term); Digestive organ expansion factor homolog; DIEXF; C1orf107; anti-DIEXF antibody
Host
Rabbit
Reactivity
Mouse, hamster (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-30 amino acids from the N-terminal region of human DEF.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
2-30
Sequence Length
756
Applicable Applications for anti-DIEXF antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(DEF Antibody (N-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DEF antibody detected the DEF protein (arrow).)
Western Blot (WB)
(DEF Antibody (N-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DEF antibody detected the DEF protein (arrow).)
Western Blot (WB)
(DEF Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the DEF antibody detected the DEF protein (arrow).)
Western Blot (WB)
(DEF Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the DEF antibody detected the DEF protein (arrow).)
Immunohistochemistry (IHC)
(DEF Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DEF Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DEF Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DEF Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of DEF Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of DEF Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-DIEXF antibody
Regulates the p53 pathway to control the expansion growth of digestive organs (By similarity).
Product Categories/Family for anti-DIEXF antibody
References
Birnbaum, S., et al. Nat. Genet. 41(4):473-477(2009)
Chen, J., et al. Genes Dev. 19(23):2900-2911(2005)
Chen, J., et al. Genes Dev. 19(23):2900-2911(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
87055
NCBI Official Full Name
digestive organ expansion factor homolog
NCBI Official Synonym Full Names
digestive organ expansion factor homolog (zebrafish)
NCBI Official Symbol
DIEXF
NCBI Official Synonym Symbols
DEF; UTP25; C1orf107; DJ434O14.5
NCBI Protein Information
digestive organ expansion factor homolog
UniProt Protein Name
Digestive organ expansion factor homolog
Protein Family
UniProt Gene Name
DIEXF
UniProt Synonym Gene Names
C1orf107; DEF
UniProt Entry Name
DIEXF_HUMAN
Uniprot Description
DEF: Regulates the p53 pathway to control the expansion growth of digestive organs. Belongs to the def family.
Protein type: Nucleolus
Chromosomal Location of Human Ortholog: 1q32.2
Cellular Component: small subunit processome; nucleolus
Molecular Function: rRNA binding
Biological Process: maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); multicellular organismal development
Research Articles on DIEXF
Similar Products
Product Notes
The DIEXF diexf (Catalog #AAA9207874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-30. The DEF Antibody (N-term) reacts with Mouse, hamster (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's DEF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the DIEXF diexf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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