Rabbit DEF Polyclonal Antibody | anti-DEF antibody

DEF, NT (DIEXF, C1orf107, DEF, Digestive organ expansion factor homolog) (AP)

Cat. Num. AAA6291585

• Gene Names: DIEXF; DEF; UTP25; C1orf107; DJ434O14.5
• Reactivity: Hamster, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DEF; Polyclonal Antibody; NT (DIEXF; C1orf107; Digestive organ expansion factor homolog) (AP); DIEXF; Digestive organ expansion factor homolog; anti-DEF antibody

• Host: Rabbit
• Reactivity: Hamster, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-DEF antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human DEF.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DEF Antibody (N-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DEF antibody detected the DEF protein (arrow).)

Western Blot (WB)

(DEF Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the DEF antibody detected the DEF protein (arrow).)

Immunohistochemistry (IHC)

(DEF Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DEF Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of DEF Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-DEF antibody

Regulates the p53 pathway to control the expansion growth of digestive organs (By similarity).

Product Categories/Family for anti-DEF antibody

Antibodies; KIAA; orf

References

Birnbaum, S., et al. Nat. Genet. 41(4):473-477(2009). Chen, J., et al. Genes Dev. 19(23):2900-2911(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 75677335
• NCBI GeneID: 27042
• NCBI Accession #: NP_055203.4
• NCBI GenBank Nucleotide #: NM_014388.6
• UniProt Accession #: Q68CQ4; O75992; Q4VY00; Q63HL9
• Molecular Weight: 87,055 Da
• NCBI Official Full Name: digestive organ expansion factor homolog
• NCBI Official Synonym Full Names: digestive organ expansion factor homolog (zebrafish)
• NCBI Official Symbol: DIEXF
• NCBI Official Synonym Symbols: DEF; UTP25; C1orf107; DJ434O14.5
• NCBI Protein Information: digestive organ expansion factor homolog
• UniProt Protein Name: Digestive organ expansion factor homolog
• Protein Family: Defensin
• UniProt Gene Name: DIEXF
• UniProt Synonym Gene Names: C1orf107; DEF
• UniProt Entry Name: DIEXF_HUMAN

Uniprot Description

DEF: Regulates the p53 pathway to control the expansion growth of digestive organs. Belongs to the def family.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 1q32.2

Cellular Component: small subunit processome; nucleolus

Molecular Function: rRNA binding

Biological Process: maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); multicellular organismal development