Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Testis Tissue; Lane2: Rat Placenta Tissue; Lane3: Mouse Testis Tissue; Lane4: Human A549 Cells.)
Rabbit Decay Accelerating Factor (DAF) Polyclonal Antibody | anti-DAF antibody
Polyclonal Antibody to Decay Accelerating Factor (DAF)
MGHHHHHHSG SEF- PHESTLI NFPSTRAPLS HKPTTVKVPG TRVKPTSHKP TEVKVPATQH VPVSKTTVRH PTRTSKDRGE SNSGGDHFIY GFVAVIVTID SIILIKTLWW TILSSNRSSD
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Testis Tissue; Lane2: Rat Placenta Tissue; Lane3: Mouse Testis Tissue; Lane4: Human A549 Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Testis Tissue; Lane2: Rat Placenta Tissue; Lane3: Mouse Testis Tissue; Lane4: Human A549 Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant DAF, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant DAF, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Intestine Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Intestine Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Pancreas Tissue.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]
Uniprot Description
CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell surface; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: cell surface; integral to plasma membrane; extracellular region; plasma membrane; lipid raft
Molecular Function: viral receptor activity; protein binding; lipid binding
Biological Process: respiratory burst; entry of virus into host cell; elevation of cytosolic calcium ion concentration; negative regulation of complement activation; regulation of complement activation; innate immune response; complement activation, classical pathway; regulation of lipopolysaccharide-mediated signaling pathway
Disease: Blood Group, Cromer System
Research Articles on DAF
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Product Notes
The DAF (Catalog #AAA2004681) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Decay Accelerating Factor (DAF) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Decay Accelerating Factor (DAF) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the DAF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- PHESTLI NFPSTRAPLS HKPTTVKVPG TRVKPTSHKP TEVKVPATQH VPVSKTTVRH PTRTSKDRGE SNSGGDHFIY GFVAVIVTID SIILIKTLWW TILSSNRSSD. It is sometimes possible for the material contained within the vial of "Decay Accelerating Factor (DAF), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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