Rabbit anti-Human DDX53 Polyclonal Antibody | anti-DDX53 antibody

DDX53, CT (DDX53, CAGE, Probable ATP-dependent RNA helicase DDX53, Cancer-associated gene protein, Cancer/testis antigen 26, DEAD box protein 53, DEAD box protein CAGE) (MaxLight 405)

Cat. Num. AAA6291513

• Gene Names: DDX53; CAGE; CT26
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: DDX53; Polyclonal Antibody; CT (DDX53; CAGE; Probable ATP-dependent RNA helicase DDX53; Cancer-associated gene protein; Cancer/testis antigen 26; DEAD box protein 53; DEAD box protein CAGE) (MaxLight 405); DEAD box protein CAGE; anti-DDX53 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DDX53.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-DDX53 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 590-619 in the C-terminal region of human DDX53, UniProt Accession #Q86TM3.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in A549 cell line lysates (35ug/lane) usingMBS6008594. This demonstrates that MBS6008594 detected the DDX53 protein (arrow).)

Related Product Information for anti-DDX53 antibody

DDX53 belongs to the DEAD box helicase family. It is expressed in testis and shows wide expression in various cancer tissues and cancer cell lines.

Product Categories/Family for anti-DDX53 antibody

Antibodies; DEAD Box Proteins

References

1. Kim, Y., et al. J. Biol. Chem. 285(34):25957-25968(2010). 2. Pinto, D., et al. Nature 466(7304):368-372(2010). 3. Kim, Y., et al. J. Microbiol. Biotechnol. 18(3):600-610(2008). 4. Lee, T.S., et al. Ann. N. Y. Acad. Sci. 1091, 218-224 (2006). 5. Shim, H., et al. Biotechnol. Lett. 28(24):2057-2063(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 55749865
• NCBI GeneID: 168400
• NCBI Accession #: NP_874358.2
• NCBI GenBank Nucleotide #: NM_182699.3
• UniProt Accession #: Q86TM3; Q0D2N2; Q6NVV4
• Molecular Weight: 71,154 Da
• NCBI Official Full Name: DEAD box protein 53
• NCBI Official Synonym Full Names: DEAD-box helicase 53
• NCBI Official Symbol: DDX53
• NCBI Official Synonym Symbols: CAGE; CT26
• NCBI Protein Information: DEAD box protein 53
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX53
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX53
• UniProt Synonym Gene Names: CAGE; CT26

NCBI Description

This intronless gene encodes a protein which contains several domains found in members of the DEAD-box helicase protein family. Other members of this protein family participate in ATP-dependent RNA unwinding. [provided by RefSeq, Sep 2011]

Uniprot Description

DDX53: This intronless gene encodes a protein which contains several domains found in members of the DEAD-box helicase protein family. Other members of this protein family participate in ATP-dependent RNA unwinding. [provided by RefSeq, Sep 2011]

Protein type: Cancer Testis Antigen (CTA); EC 3.6.4.13; Helicase

Chromosomal Location of Human Ortholog: Xp22.11

Cellular Component: cytoplasm; nucleolus

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; RNA binding

Biological Process: RNA secondary structure unwinding