Rabbit anti-Human DDX50 Polyclonal Antibody | anti-DDX50 antibody

DDX50 (ATP-dependent RNA Helicase DDX50, DEAD Box Protein 50, Gu-beta, Nucleolar Protein Gu2, MGC3199) (HRP)

Cat. Num. AAA6375912

• Gene Names: DDX50; GU2; GUB; mcdrh; RH-II/GuB
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DDX50; Polyclonal Antibody; DDX50 (ATP-dependent RNA Helicase DDX50; DEAD Box Protein 50; Gu-beta; Nucleolar Protein Gu2; MGC3199) (HRP); anti-DDX50 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DDX50.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DDX50 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human DDX50, aa1-737 (NP_076950.1).
• Immunogen Sequence: MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEKSETRENGVTDDLDAPKAKKSKMKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DDX50 rabbit polyclonal antibody. Western Blot analysis of DDX50 expression in human kidney.)

Western Blot (WB)

(DDX50 rabbit polyclonal antibody. Western Blot analysis of DDX50 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of DDX50 expression in transfected 293T cell line by DDX50 polyclonal antibody. Lane 1: DDX50 transfected lysate (82.6kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-DDX50 antibody

Antibodies; DEAD Box Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13129006
• NCBI GeneID: 79009
• NCBI Accession #: NP_076950.1
• NCBI GenBank Nucleotide #: NM_024045.1
• UniProt Accession #: Q9BQ39; Q5VX37; Q8WV76; Q9BWI8
• Molecular Weight: 82,565 Da
• NCBI Official Full Name: ATP-dependent RNA helicase DDX50
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
• NCBI Official Symbol: DDX50
• NCBI Official Synonym Symbols: GU2; GUB; mcdrh; RH-II/GuB
• NCBI Protein Information: ATP-dependent RNA helicase DDX50; gu-beta; DEAD box protein 50; nucleolar protein GU2; RNA helicase II/Gu beta; malignant cell derived RNA helicase
• UniProt Protein Name: ATP-dependent RNA helicase DDX50
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DDX50
• UniProt Entry Name: DDX50_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX50: an RNA helicase of the DEAD-box family.

Protein type: EC 3.6.4.13; RNA-binding; Helicase; Nucleolus

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: membrane; nucleolus; plasma membrane

Molecular Function: helicase activity; ATP binding

Biological Process: metabolic process