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Western Blot (WB) (DDX50 rabbit polyclonal antibody. Western Blot analysis of DDX50 expression in human kidney.)

Rabbit anti-Human DDX50 Polyclonal Antibody | anti-DDX50 antibody

DDX50 (ATP-dependent RNA Helicase DDX50, DEAD Box Protein 50, Gu-beta, Nucleolar Protein Gu2, MGC3199) APC

Gene Names
DDX50; GU2; GUB; mcdrh; RH-II/GuB
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DDX50; Polyclonal Antibody; DDX50 (ATP-dependent RNA Helicase DDX50; DEAD Box Protein 50; Gu-beta; Nucleolar Protein Gu2; MGC3199) APC; anti-DDX50 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human DDX50.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-DDX50 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human DDX50, aa1-737 (NP_076950.1).
Immunogen Sequence
MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEKSETRENGVTDDLDAPKAKKSKMKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD
Conjugate
APC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DDX50 rabbit polyclonal antibody. Western Blot analysis of DDX50 expression in human kidney.)

Western Blot (WB) (DDX50 rabbit polyclonal antibody. Western Blot analysis of DDX50 expression in human kidney.)

Western Blot (WB)

(DDX50 rabbit polyclonal antibody. Western Blot analysis of DDX50 expression in HeLa.)

Western Blot (WB) (DDX50 rabbit polyclonal antibody. Western Blot analysis of DDX50 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of DDX50 expression in transfected 293T cell line by DDX50 polyclonal antibody. Lane 1: DDX50 transfected lysate (82.6kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of DDX50 expression in transfected 293T cell line by DDX50 polyclonal antibody. Lane 1: DDX50 transfected lysate (82.6kD). Lane 2: Non-transfected lysate.)
Product Categories/Family for anti-DDX50 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,565 Da
NCBI Official Full Name
ATP-dependent RNA helicase DDX50
NCBI Official Synonym Full Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
NCBI Official Symbol
DDX50
NCBI Official Synonym Symbols
GU2; GUB; mcdrh; RH-II/GuB
NCBI Protein Information
ATP-dependent RNA helicase DDX50; gu-beta; DEAD box protein 50; nucleolar protein GU2; RNA helicase II/Gu beta; malignant cell derived RNA helicase
UniProt Protein Name
ATP-dependent RNA helicase DDX50
UniProt Gene Name
DDX50
UniProt Entry Name
DDX50_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX50: an RNA helicase of the DEAD-box family.

Protein type: EC 3.6.4.13; RNA-binding; Helicase; Nucleolus

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: membrane; nucleolus; plasma membrane

Molecular Function: helicase activity; ATP binding

Biological Process: metabolic process

Research Articles on DDX50

Similar Products

Product Notes

The DDX50 ddx50 (Catalog #AAA6375909) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDX50 (ATP-dependent RNA Helicase DDX50, DEAD Box Protein 50, Gu-beta, Nucleolar Protein Gu2, MGC3199) APC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDX50 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DDX50 ddx50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX50, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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