Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat DDX5/p68 RNA helicase Polyclonal Antibody | anti-DDX5 antibody
Goat anti-DDX5/p68 RNA helicase Antibody
Immunohistochemistry: In paraffin embedded Human Prostate shows nuclear staining of the secretory cells in the glands. Recommended concentration, 2-5ug/ml. Data obtained using a previous batch.
Western Blot: Approx 70kDa band observed in nuclear lysates of cell lines NIH3T3, A431, HeLa and Jurkat (calculated MW of 69.1kDa according to NP_004387.1). Recommended concentration: 0.3-1ug/ml.
Immunofluorescence: A customer has reported successful staining at a dilution of 1: 100 on HT-1080 cells with a Rhodamine conjugated secondary antibody. Data obtained using a previous batch.
Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data obtained using a previous batch.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data obtained using a previous batch.)
NCBI and Uniprot Product Information
NCBI Description
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Uniprot Description
DDX5: a putative ATP-dependent RNA helicase. A proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Belongs to the DEAD box helicase family. DDX5/DDX17 subfamily. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.
Protein type: Spliceosome; Helicase; RNA-binding; RNA splicing; EC 3.6.4.13; Nuclear receptor co-regulator; Nucleolus
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: membrane; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex
Molecular Function: androgen receptor binding; ATP binding; ATP-dependent RNA helicase activity; calcium-dependent protein binding; calmodulin binding; enzyme binding; estrogen receptor binding; protein binding; RNA helicase activity; transcription coactivator activity
Biological Process: cell growth; mRNA transcription; negative regulation of transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; positive regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of alternative nuclear mRNA splicing, via spliceosome; regulation of osteoblast differentiation; regulation of viral genome replication; rhythmic process; RNA secondary structure unwinding
Research Articles on DDX5
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Product Notes
The DDX5 ddx5 (Catalog #AAA420270) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DDX5/p68 RNA helicase Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's DDX5/p68 RNA helicase can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunohistochemistry: In paraffin embedded Human Prostate shows nuclear staining of the secretory cells in the glands. Recommended concentration, 2-5ug/ml. Data obtained using a previous batch. Western Blot: Approx 70kDa band observed in nuclear lysates of cell lines NIH3T3, A431, HeLa and Jurkat (calculated MW of 69.1kDa according to NP_004387.1). Recommended concentration: 0.3-1ug/ml. Immunofluorescence: A customer has reported successful staining at a dilution of 1: 100 on HT-1080 cells with a Rhodamine conjugated secondary antibody. Data obtained using a previous batch. Researchers should empirically determine the suitability of the DDX5 ddx5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PMIGYPMPTG YSQ. It is sometimes possible for the material contained within the vial of "DDX5/p68 RNA helicase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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