SDS-PAGE
CDC7 recombinant protein
CDC7 recombinant protein
Gene Names
CDC7; Hsk1; CDC7L1; HsCDC7; huCDC7
Applications
Western Blot
Synonyms
CDC7; CDC7 recombinant protein; CDC7L1; HsCDC7; Hsk1; huCDC7; MGC117361; MGC126237; MGC126238
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Length
3222
Applicable Applications for CDC7 recombinant protein
Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human CDC7 was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for CDC7 recombinant protein
Recombinant full-length human CDC7 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: CDC7 is a cell division cycle protein that is critical for the G1/S transition and initiation of DNA replication during the cell division cycle. Overexpression of CDC7 gene product may be associated with neoplastic transformation for some tumors. Inhibition of CDC7 in cancer cells impairs progression through S phase, inducing a p53-independent apoptotic cell death, whereas in normal cells, it does not affect cell viability (1). Inhibition of CDC7 kinase activity in cancer cells restricts DNA replication and induces apoptosis. CDC7 phosphorylates the minichromosome maintenance protein 2 (Mcm2), a component of the DNA replicative helicase needed for genome duplication (2).
Scientific Background: CDC7 is a cell division cycle protein that is critical for the G1/S transition and initiation of DNA replication during the cell division cycle. Overexpression of CDC7 gene product may be associated with neoplastic transformation for some tumors. Inhibition of CDC7 in cancer cells impairs progression through S phase, inducing a p53-independent apoptotic cell death, whereas in normal cells, it does not affect cell viability (1). Inhibition of CDC7 kinase activity in cancer cells restricts DNA replication and induces apoptosis. CDC7 phosphorylates the minichromosome maintenance protein 2 (Mcm2), a component of the DNA replicative helicase needed for genome duplication (2).
Product Categories/Family for CDC7 recombinant protein
References
1. Montagnoli, A. et al: Targeting cell division cycle 7 kinase: a new approach for cancer therapy. Clin Cancer Res. 2010 Sep 15;16(18):4503-8.
2. Chuang, L C. et al: Phosphorylation of Mcm2 by Cdc7 promotes pre-replication complex assembly during cell-cycle re-entry. Mol Cell. 2009 Jul 31;35(2):206-16.
2. Chuang, L C. et al: Phosphorylation of Mcm2 by Cdc7 promotes pre-replication complex assembly during cell-cycle re-entry. Mol Cell. 2009 Jul 31;35(2):206-16.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~94 kDa
NCBI Official Full Name
Homo sapiens cell division cycle 7 (CDC7), transcript variant 1, mRNA
NCBI Official Synonym Full Names
cell division cycle 7
NCBI Official Symbol
CDC7
NCBI Official Synonym Symbols
Hsk1; CDC7L1; HsCDC7; huCDC7
NCBI Protein Information
cell division cycle 7-related protein kinase; CDC7-related kinase; cell division cycle 7 homolog; cell division cycle 7-like protein 1; CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1
UniProt Protein Name
Cell division cycle 7-related protein kinase
Protein Family
UniProt Gene Name
CDC7
UniProt Synonym Gene Names
CDC7L1; CDC7-related kinase; HsCdc7; huCdc7
UniProt Entry Name
CDC7_HUMAN
NCBI Description
This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008]
Uniprot Description
Function: Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3. Ref.9
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium.
Subunit structure: Forms a complex with either DBF4/DBF4A or DBF4B, leading to the activation of the kinase activity.
Subcellular location: Nucleus.
Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.Contains 1 protein kinase domain.
Research Articles on CDC7
Similar Products
Product Notes
The CDC7 cdc7 (Catalog #AAA515101) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human CDC7 was expressed by baculovirus in Sf9 insect cells. AAA Biotech's CDC7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CDC7 cdc7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC7, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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