Rabbit DDX4/MVH Polyclonal Antibody | anti-DDX4 antibody

Anti-DDX4/MVH Antibody

Cat. Num. AAA1753042

• Gene Names: DDX4; VASA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: DDX4/MVH; Polyclonal Antibody; Anti-DDX4/MVH Antibody; Probable ATP-dependent RNA helicase DDX4; DEAD box protein 4; Vasa homolog; DDX4; VASA; anti-DDX4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 690

• Applicable Applications for anti-DDX4 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Mouse, Rat); IHC-P (Embedded Section): 0.5-1ug/ml (Mouse, Rat); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
• Immunogen: E Coli-derived human DDX4/MVH recombinant protein (Position: D3-D666).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-DDX4 antibody

Description: Rabbit IgG polyclonal antibody for DDX4/MVH detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human; Mouse; Rat.

Background: DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.

References

1. Abe, K., Noce, T. A DEAD-family protein gene, Ddx4, encoding a murine homolog of Drosophila vasa maps to the distal end of mouse chromosome 13. Mammalian Genome 8: 622-623, 1997.
2. Castrillon, D. H., Quade, B. J., Wang, T. Y., Quigley, C., Crum, C. P. The human VASA gene is specifically expressed in the germ cell lineage. Proc. Nat. Acad. Sci. 97: 9585-9590, 2000.
3. Chu, D. S., Liu, H., Nix, P., Wu, T. F., Ralston, E. J., Yates, J. R., III, Meyer, B. J. Sperm chromatin proteomics identifies evolutionarily conserved fertility factors. Nature 443: 101-105, 2006.

NCBI and Uniprot Product Information

• NCBI GI #: 216548263
• NCBI GeneID: 54514
• NCBI Accession #: NP_001136021.1
• NCBI GenBank Nucleotide #: NP_001136021.1
• UniProt Accession #: Q9NQI0
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX4 isoform 2
• NCBI Official Synonym Full Names: DEAD-box helicase 4
• NCBI Official Symbol: DDX4
• NCBI Official Synonym Symbols: VASA
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX4
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX4
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX4
• UniProt Synonym Gene Names: VASA
• UniProt Entry Name: DDX4_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]