Rabbit anti-Human DDX39 Polyclonal Antibody | anti-DDX39 antibody

DDX39, ID (Y265) (DDX39A, DDX39, ATP-dependent RNA helicase DDX39A, DEAD box protein 39, Nuclear RNA helicase URH49) (Biotin)

Cat. Num. AAA6291444

• Gene Names: DDX39A; BAT1; DDXL; BAT1L; DDX39; URH49
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: DDX39; Polyclonal Antibody; ID (Y265) (DDX39A; ATP-dependent RNA helicase DDX39A; DEAD box protein 39; Nuclear RNA helicase URH49) (Biotin); DDX39A; Nuclear RNA helicase URH49; anti-DDX39 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DDX39 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DDX39 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256~286 amino acids from the Central region of human DDX39.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DDX39 Antibody (Center Y265) in HepG2 cell line lysates (35ug/lane). DDX39 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of DDX39 (arrow) using rabbit polyclonal DDX39 Antibody (Center Y265). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DDX39 gene.)

Related Product Information for anti-DDX39 antibody

DDX39 is a member of the DEAD box protein family. These proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Product Categories/Family for anti-DDX39 antibody

Antibodies; DEAD Box Proteins

References

Pryor A., Tung L., Yang Z.Nucleic Acids Res. 32:1857-1865(2004). Lehner B., Semple J.I., Brown S.E.Genomics 83:153-167(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 21040371
• NCBI GeneID: 10212
• NCBI Accession #: NP_005795
• NCBI GenBank Nucleotide #: NM_005804.3
• UniProt Accession #: O00148; Q8N5M0; Q9BVP6; Q9H5W0; B1Q2N1
• Molecular Weight: 31.0 kDa (274 aa)
• NCBI Official Full Name: ATP-dependent RNA helicase DDX39A
• NCBI Official Synonym Full Names: DExD-box helicase 39A
• NCBI Official Symbol: DDX39A
• NCBI Official Synonym Symbols: BAT1; DDXL; BAT1L; DDX39; URH49
• NCBI Protein Information: ATP-dependent RNA helicase DDX39A
• UniProt Protein Name: ATP-dependent RNA helicase DDX39A
• UniProt Gene Name: DDX39A
• UniProt Synonym Gene Names: DDX39

NCBI Description

This gene encodes a member of the DEAD box protein family. These proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is thought to play a role in the prognosis of patients with gastrointestinal stromal tumors. A pseudogene of this gene is present on chromosome 13. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]

Uniprot Description

Isoform 1: Involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus.