Rabbit anti-Human PRAMEF14 Polyclonal Antibody | anti-PRAMEF14 antibody

PRAMEF14, ID (PRAMEF14, PRAME family member 14) (AP)

Cat. Num. AAA6336918

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRAMEF14; Polyclonal Antibody; ID (PRAMEF14; PRAME family member 14) (AP); PRAME family member 14; anti-PRAMEF14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PRAMEF14 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PRAMEF14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-242 amino acids from the Central region of human PRAMEF14.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PRAMEF14 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PRAMEF14 antibody detected the PRAMEF14 protein (arrow).)

Related Product Information for anti-PRAMEF14 antibody

Melanoma antigen preferentially expressed in tumors is a protein that in humans is encoded by the PRAME gene.1,2,3 which encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene.3

Product Categories/Family for anti-PRAMEF14 antibody

Antibodies; PRAME Proteins

References

Gregory, S.G., et al. Nature 441(7091):315-321(2006). General References: 1. Ikeda H, Lethe B, Lehmann F, van Baren N, Baurain JF, de Smet C, Chambost H, Vitale M, Moretta A, Boon T, Coulie PG (Mar 1997). "Characterization of an antigen that is recognized on a melanoma showing partial HLA loss by CTL expressing an NK inhibitory receptor". Immunity 6 (2): 199-208. doi:10.1016/S1074-7613(00)80426-4. PMID 9047241. 2. Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP, et al. (Dec 1999). "The DNA sequence of human chromosome 22". Nature 402 (6761): 489-95. doi:10.1038/990031. PMID 10591208. 3. "Entrez Gene: PRAME preferentially expressed antigen in melanoma".

NCBI and Uniprot Product Information

• NCBI GI #: 153792140
• NCBI GeneID: 729528
• NCBI Accession #: NP_001093324.1
• NCBI GenBank Nucleotide #: NM_001099854.1
• UniProt Accession #: Q5SWL7
• Molecular Weight: 49,433 Da
• NCBI Official Synonym Full Names: PRAME family member 14
• NCBI Official Symbol: PRAMEF14
• NCBI Protein Information: PRAME family member 14
• UniProt Protein Name: PRAME family member 14
• Protein Family: PRAME family
• UniProt Gene Name: PRAMEF14

Uniprot Description

PRAMEF14: Belongs to the PRAME family.

Chromosomal Location of Human Ortholog: 1p36.21

Biological Process: negative regulation of apoptosis; negative regulation of cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation